Wham! Line/Web Comics

"Klaus: "What - Judy!""
 * Girl Genius: "Oh, and I saw the Baron a bit when I was on Castle Wulfenbach. But I had to run away when everybody found out I was a Heterodyne."
 * "I believe the ignorant call them slaver wasps."
 * "Yes...But I vas starting to like her."
 * Or probably the earliest,

"Sheena: (to Radd) "Besides, if you'd died, how could I ?" Sheena: ".""
 * "I am sorry, mistress...I...I cannot." Spoken by, letting the heroine just how screwed the situation is.
 * And: "SCREEE! GROWR! SSSSSS! SSSSSS!"
 * And following that: "She didn't have to. You were infected before you even met her."
 * Megatokyo: "If I sleep with you, will you go away?"
 * Kid Radd: "No no no, Crystal has left the building..."
 * Sheena gets a few.

": "Torg, it was me.""
 * City of Reality: "Take me back. Now."
 * The Space Between: "Come inside. People might think you're an actual hobo."
 * Sluggy Freelance:

"Riff: How long have I been here? Rammer:"
 * "It was about that time Aylee announced she was dying."
 * "But you can be at peace, for we are your people, Aylee. You are finally home."
 * And then in 4U City Red:

"Muut: Annie:"
 * Gunnerkrigg Court:

"Reynardine:"

"Coyote:"

"Antimony:"

"Tarquin: First Prisoner: Second Prisoner: Tarquin: First prisoner: Speaking of names, we haven't properly introduced ourselves. Second Prisoner: They used to call me, but three years of malnutrition will."
 * The Order of the Stick Strip #758 is somewhat unique in that several people deliver the Wham.

"Horace: How long do you think you've been here? In the ? Roy: I dunno, 12 hours? Maybe 14? Why? Horace: Roy...."
 * "...Now I will kill your children."
 * From #498:

"AA: banished fr0m the universe we left behind AA: and yet in being denied the ultimate reward AA: we w0uld be barred fr0m entry AA: int0 the universe we created."
 * Out There gives us this (little spoileriffic). After Miriam and Clayton have been bantering, and the former has made it perfectly clear that that's not going to happen, and the latter has been accepting of that: "Anyway, don't you think any of these losers wouldn't flock to see you [work as a stripper]? " The afterward serves the same general purpose.
 * A lengthy time-travel arc in Narbonic is turned on its head with a single innocuous line.
 * And in its final month, courtesy of a young time-traveller: "And I think, in the end, can find some kind of happiness." The character being spoken to didn't even realize the bomb that had just been dropped until a few seconds later -- you can almost hear the Record Needle Scratch.
 * Homestuck gets more than a few. In chronological order:
 * Courtesy of Aradia Megido:

"TG: im pretty sure it was TG: like TT: ? TG: a plea for help"
 * Dave listens to the Horrorterrors.

"TG: what you mean a troll TT: No. TT: It's a man who exists in another universe. TT: He wants to die."
 * And then Rose reveals the identity of her informant:

"GG: because he said hes my grandson"
 * A pretty big one came buried under lots of text here

"GA: He Is Not Responsible For Just One Aspect Of The Universe You Create GA: He's Responsible For All Of Them GA: Bilious Slick Is Your Universe"
 * "While a timer counts down with no particular clock stepping forward to claim it...The most important character in Homestuck fondly regards the miracle of a new beginning."
 * One word shattered the expectations of fans: DEAD. It also overlaps with Unsound Effect.
 * Kanaya informs Jade on the importance of the Genesis Frog:

"CA: fight him CA: are you fuckin nuts CA: i slaughtered enough angels to knoww my limits and wwhere i stand against the lord of all angels they prophesized CA: of course im not gonna fight him i stand no chance in hell against that guy CA: im goin to join him"
 * And with this line from Eridan, everything goes to hell:

"TC: shut up."
 * Then after a series of shocking events: "Don't turn your back on the body."
 * And then:

"GT: This was not the suit i was wearing when i died."
 * "Pardon me while I let Jack inside."
 * Doc Scratch gets a few of these during his intermission. Sorted by date:
 * 5/31/11: "With as little fanfare, he seized the opportunity to follow the Thief's trail quickly before it dissipated, and destroyed their hideout in the veil."
 * 6/8/11: "What sort of story would this be, with our Knight and Seer made to stay cadavers? Certainly not one the alpha timeline would allow. And not one she'd allow either."
 * 6/19/11: "YOU'RE SUPPOSED TO KILL HER."
 * When Dreambubble John finally begins to remember his death:

"color:"
 * Davesprite explains to Jade his second encounter with Hephaestus:

"pipefan413: She knew the baroness was not human! :B"
 * From Nannasprite, on the identity of her adopted mother, Betty Crocker:

"PCG: I GAVE YOUR WHOLE UNIVERSE CANCER, JADE."
 * Karkat apologizes:

"TC: AND I WENT AND MADE YOUR UNIVERSE... TC: terminal. Bo)"
 * Betty Crocker: @turntechGodhead Please enjoy. Mr. Strider. http://tinyurl.com/MoThErFuCkInMiRaClEs
 * We find out why Gamzee is "the most important character in Homestuck":

"S u c k e r s."
 * Quoth Doc Scratch about an early set of Alternians: "The heroes chose to accept this bargain, and scratched their session."
 * A little later, same conversation: "But in addition to losing their memories of everything that had happened before the scratch, there was another catch for the failed heroes. In the new reality, they would not serve as the heroes."
 * And a final one from Scratch, probably the biggest of all, at the EOA5 flash, :

"color:"
 * uranianUmbra [UU] began cheering gutsyGumshoe [GG] at 11:17
 * In an otherwise silly and hilarious conversation with a 15-year-old drunk:

"color:"
 * "The Page is dead. Our hope is lost."
 * When John asks how long it will take him and Jade to cross the Yellow Yard and arrive in the new session:

"color:"
 * "You say it's all fair game now that the kingdom's under new management. The new boss ain't opposed to taking some shrewd tactical shortcuts."
 * Followed by: "He says it's probably because she wears the most grand and luxurious fluffy hat he's ever seen. You say you don't think that's a hat. You think it's something called 'hair.'"
 * In a conversation with Dirk Strider, Roxy drops this conversational bomb, about her "upbringin"

"color: color:"
 * In a dream bubble, Terezi has a chat with Vriska. Only to learn something unexpected.

"color:"
 * And with this line, humanity's fate is made significantly less ambiguous:

"Yuki: promise we'll be like this forever..."
 * uu: I ALREADY KILLED HER!!!!!
 * Mortifer: "That's...Well, that's impossible...There was only one demon with that name, and he died off hundreds of years ago."
 * "The new leader of the black market, Vlademyre Hynner."
 * "We have a demon of our own."
 * "That's when I started to realize something. This entire business had to be crushed, and all its people have to pay for what they've done."
 * "Yes, he worked at Sintec, but your research lacked greatly. Sintec doubles as a mercenary company, the idea of the "exterminator" company was simply a cover for the real thing. Joey had taken over the presidency after the unfortunate accident of the former president."
 * Haru-Sari: "Come on, Cygnet, this isn't even your room!"
 * Ménage à 3: (NSFW)

"Sara: "Will you just! Kiss her! Already?""
 * Penny and Aggie
 * Early on,

"Penny: "You wanna...pretend a little more for a minute?""
 * Which much later led to...

" Chaos : Very well. You want to make a difference? You want a world you can freely participate in?"
 * Questionable Content: "Marten, do you like me?" As Faye herself put it, "like interrupting an intricate waltz with a sledgehammer to the knee."
 * Also, much later, "...Well. I guess that's it, then," turning a pretty standard Dora/Marten arc into a Wham! Episode.
 * El Goonish Shive: "It was real. The monster was a golem created by one who must be found. The girl is a good Samaritan who can fly. What was seen is what was."
 * Before that, near the end of Sister II:

"Nicole: "Admittedly, calling someone an attempted murderer is harsh, but tell me, Mr. Terrell, what were you doing before you were hired by GPF Software?""
 * General Protection Fault

"Nick: "So now that that’s done and over with, can we talk about ""
 * Later on, not so much because of the secret discussed but because Nick is aware of it.

"Sharon: Dex and I will be staying at his folks' place for a while. Dexter: Yeah, they can still hardly believe it...I’ve finally got a girlfriend."
 * When Fooker goes to his ex-girlfriend Sharon to apologize.

"Yoshi: Just as well, I guess. She’s too wrapped up in her loser boyfriend. He’ll be back any minute now..."
 * While Trudy and Yoshi are discussing Ki, and Trudy is hesitant to go see her.

"Aaryana: "Destania must be so proud to have such a wonderful and kind incubus like you for a son!" Abel (reading Jyrras's mind): "Lesse... Dan...D an...friends since childhood...came to rescue him... have had a slight crush on him since...""
 * Looking for Group: "He's not dead."
 * And, on a related note, "Ow?"
 * Dan and Mab's Furry Adventures:
 * Dan and Mab's Furry Adventures:

"M.A.C.E. (looking up Abel): "Father: Aniz Siar (Cubi) / Born: Unknown / Status: Deceased (1964 Dekith) / [Killed by Quintinga Ti'fona].""
 * Or on a much more ominous note.

"Mab: "It is hard enough knowing .""
 * Even more ominous:

"Otra:"
 * Starfighter: "Gentlemen, this is a time sensitive matter. Therefore I shall be brief. "
 * Girly. Courtesy of Otra.

"Kevin: (to the rabbit that saved Kell when she went to look for his parents) Thanks, Dad."
 * Something*Positive: "...But I'm not drunk now, Jason."
 * ".... oh. Oh."
 * Kevin and Kell

"Dorothy: Is that another request for an arranged marriage with Coney? Kevin: Yes, Mom...an archaic, backward tradition! Dorothy: I know, it's too bad the one I set up for you in infancy didn't work out."
 * This exchange

"Kevin: But I’m not up for re-election for years! Intruder: Check that. You’re facing a recall."
 * After the intruder identifies himself as a political operative for Kevin's Rabbit Council opponent.

"Papa Kindle: But if neither of us is the killer, then who is?
 * When Rudy asks who would be unlucky enough to have to give Vin Vulpen a tail transplant, Lindesfarne holds a ruler at his tail and says "Four inches should do it."
 * "Kell...you...you won. You're the new CEO of Herd Thinners!"
 * After it's revealed that the evidence connecting Kevin's father and Angelique to killing Sid was done to them by the other in the hopes of Framing the Guilty Party, this exchange happens.
 * I'd say"

"Ethan: Well lookie there, we have a winner."
 * Dominic Deegan had one: "David Johann, the King of Callan"
 * Shortpacked!
 * A guessing game ends.

"nice!Mike: So you plan to keep me inebriated to make me the perfect boyfriend? I hate to say. It's been done. And it didn't end well. Amber: No, I want you to date me sober."
 * The beginning of a disturbing relationship.

"Roz: Not since-- no, look, hey, LEAVE, okay before-- Robin: Before what? Before I get the stink of worldwide hero all over the place? Roz: No, before... Leslie: Before I find you."
 * World peace at the ultimate price.

"Amber: Since when are you helpful? Mike: Since being "helpful" means sending people to go wait in four-hour lines for stuff that's likely to sell out just as they reach the booth. Amber: Wow. I'm marrying the devil. Mike: Hasbro's making this way too easy for me. (the comic appears to be over, then suddenly, one extra panel at the very bottom...) Mike: Wait, what?"
 * At the end of a fairly typical filler week, we see Mike and Amber at Comic-Con:

"Leslie: Bull. Are you telling me your vomitfest was just for show? Amber: S'no big deal. Just been feeling extra nauseous this week. Leslie: Um. Amber: What?"
 * After the proposal...

"Chrii: "Guys, Manya did lie. Wrecker didn't have the idol.""
 * Survivor Fan Characters:
 * Season 7:

"Jeff Probst: "Chrii is playing the Hidden Immunity Idol. All votes for Chrii do not count. I'll read the votes.""
 * Followed quickly by:

"Cherman: "You see, there is one other tool I have. It's a mini voice recorder that Brenton and Madison accidentally made removable from my body. ""
 * Season 9:

"Natasha: I've also studied the physics code for the Internet and over half the powers you told me are impossible. IM. POSSIBLE."
 * Fans: "There is more than one being in the universe who desires souls."
 * "Television?"
 * Electric Wonderland ended its sixth story by having NJ try to call police to arrest Aerynn Arlia. The cop he talked to, Natasha Wing, refused to believe NJ's report, because she never encountered Aerynn in all her years of crimefighting. It sounds like a foolish reason not to take any action, until...

"Ceyla: So, Sarah. Your pre-print on the scientific journals stated your discovery of Pandora could reshape the current view of the history of evolution, do you really think she is that important of a discovery? Sarah: Yes. Beat Ceyla: There is an online movement claiming your videos and your discovery are a hoax, do you believe the scientific community can look past these allegations? Sarah: Yes. Ceyla: Many fans of Pandora are concerned for her safety in this school, can you reassure our viewers that no harm will come to her in the name of science? Sarah: Yes. Ceyla: That was "good", but maybe you could elaborate a little more? I feel like I could get a livelier interview out of Pandora. *to Pandora* What do you think, cutie? Would you like your own interview? Pandora: Yes."
 * Later, Trawn and NJ discover that the government's magic-seeking equipment never detects Aerynn's brand of magic. NJ then asked if this meant they can't find anyone who practices the powers as Aerynn, so she answered, "No one has my powers, Nate!" He subsequently demanded to know exactly how powerful she is. She admitted, "I don't know...Because there are a lot of things I'm afraid to try!" This sounds especially surprising after she spent nearly all of the previous comics performing actions possible only through Rule of Funny.
 * Dissonance: The setting is an entirely ordinary (but fictional) university. The main characters have discovered an unusual, undocumented kind of animal, they've named "Pandora". They've discovered she has a number of unusual traits; she can stand on two legs, her mane glows in the dark, and she cares for injured rodents, despite resembling a cat. A reporter hears about it;

"Sam: What do you even have to cry about? You're rich! I only get a $75000 allowance! You don't even take advantage of it! You wear the same earrings ALL THE TIME! That's so LAME! Princess Pi: They're stuck to my face. Sam: What? Huh? Princess Pi: The day I found out I was indestructible was the day I got my ears pierced. It took them three hours to manage to make a hole big enough, and once these were in, I couldn't remove them. That was when I was five. It was my mother, Queen Isosceles, who took me there. (She points at a nearby statue) Princess Pi: That statue over there? That's her...But now she's dead! My mother was killed in the war!"
 * Princess Pi once featured two in the same conversation:

"Maddy: "I don't mind.""
 * Too Much Information:

"Zexion: Everything you love will have my dick in it!"
 * Ansem Retort:

"Rice Boy: You have traveled long and far - you have reached the iron tower - you have changed form... thrice."
 * Rice Boy: Not so much the line itself, as who's saying it and who they're saying it to: