Ratchet and Clank: All 4 One/Funny

"Qwark: You did the right thing, Doctor!
 * In the first cutscene, right before Lawrence re-animates the Light-Eating Z'Grute, Nefarious screams in his ear like normal...only with a megaphone.
 * After  Qwark insists that Nefarious did the right thing. Nefarious's response?

Nefarious: Get bent!"

"Nefarious: Can I kill him [Qwark] now?
 * It was (sort of) featured in the trailer, but this exchange was great:

Ratchet: No.

Nefarious: I can make it look like an accident!

Ratchet: (Beat) How?

Clank: Ratchet!"

"Qwark: Listen Doctor, I completely respect the fact that you're... insane."
 * There are four simple words that are almost guaranteed to make you crack a smile: Doctor Croid:

"Ratchet: Well, Dr. Croid seems to be doing better. Still keeps feeding me those treats... but otherwise, he seems surprisingly sane."
 * This is revisited at the game's end, too:

"Clank: Ratchet! You cannot pilot a weaponized drone into Luminopolis!
 * There's also the Octonok Cay Tharpod, who tempted fate by saying that he was going to live the rest of his life free of tragic irony. There's a surprisingly long delay between this line and any further action, and you almost think that he's going to get away with it. . After scaling the lighthouse and beating the King Sepiad, the player(s) have probably written him off for dead, but as soon as the Sepiad keels over,
 * The ending is is no slouch, either. Cronk and Zephyr finally make it down to Magnus after receiving aid . When someone raises the question as to how they're going to leave Magnus, Ratchet turns toward Ephemeris and promptly announces that he'll drive.
 * Right after that, we're treated to this exchange:

Ratchet: Relax, pal-- I pilot weaponized drones into Luminopolis twice a week. It's like a ten bolt fine and a course that you have to take online. Qwark can pardon us.

Qwark: Perks of the job!

Clank: Oh, why do I even bother?"

"Croid: What planet? Who the heck are you people?"
 * There's also a seemingly-random line coming from Croid around that same time:

"Qwark: Well I don't mean to brag, but I did modify my crochitizer with a vibrate function that I like to call "The Happy Platypus""
 * Qwark's offhand comment to Nefarious about "Two guys hugging it out" is made great by the awkward silence that follows it-- particularly after one Ho Yay filled scene right before Uzo City.
 * There's a similar moment after the party defeats Commander Spog and Clank says that he knows someone who can fix the Commander if he's willing to help them. Qwark assumes that Clank is talking about him, and offers a line of dialogue that falls somewhere between CMoF and Brain Bleach material.

"Qwark: Hey Zeph! You seen Dr. Nefarious?
 * The others just stare at him until Clank finally says that he meant Nefarious.
 * The best part is Clank is the only one who looks genuinely disgusted. Ratchet and Nefarious have their hands to their chin as though they're contemplating it.
 * Qwark's half-assed attempts at finding Nefarious in the intro video. Zephyr goes wandering through the background (of Polaris's Oval Office) and...

Zephyr: Nope!

Qwark: See? The guy's a ghost!"

"Ratchet: Leave the hero stuff to the other guys.
 * The whole thing wasn't included in the teaser trailer, but it's best when seen in its completion:

Kip Darling: Like Galactic President Qwark?

Ratchet: (Beat) Yeah. Or... anyone else.

Clank: Ratchet...

Ratchet: (making an unenthusiastic effort to quiet himself) It's not like I voted for the guy..."

"Qwark: You've done some underhanded things in your time, but this beats all! I'm starting to think there may not even be an Inter-Galactic Tool of Justice Award!
 * One of Qwark's Comically Missing the Point moments from early in the game:

Ratchet: Ladies and gentlemen, your president."

"Cronk: I detect copious amounts of whippersnapperism!"
 * Nefarious following the others into the Deadgrove because the elder Tharpod's bright pink creature creeped him out.
 * After Cronk and Zephyr  Cronk lets out this line:

"Ratchet: You are such a noob."
 * Die too many times around Ratchet?

"Ratchet: I'm impressed Nefarious. You did good. *offers fist*
 * Particularly hilarious if you're playing as him in single player. You hear him yelling, distressed, for Clank over and over... and then the above line completely blindsides you.
 * Nefarious

Nefarious: *begrudgingly bumps fists*"

"Ratchet:

Dr. Croid:

Qwark:"

"Qwark: For our own survival, and for drama, we must set aside our petty squabbles and act together as a singular unit!"
 * Normally, in Single-Player mode, if you play as Ratchet, Nefarious or Qwark, a CPU Clank will ride on your back like a backpack traditionally. However, if you play as Clank, Qwark will be your backpack instead.
 * Maybe it's the delivery, but this hammy line is always good for a laugh.

"Qwark: Hmm, a letter... (Gasps happily) And it's been written in macaroni! "Dear Lombax. These Vac-Us will come in handy. Love Susie."
 * "Fatty McMouth-Breather".
 * The team discovers Vac-Us.

(Qwark turns on a Vac-U, and ends up suctioning Nefarious to it!)

Nefarious: (Trying to pull himself free) Let me go, you half-wit!

Qwark: I'm trying! Maybe if I just- (Pushes button on Vac-U and sends Nefarious flying. Cringes as a loud crash offscreen occurs.)"