Skullgirls/Nightmare Fuel

"Valentine: "Tell me where it hurts.""
 * Peacock. A Child Slave turned into a highly disturbed killing machine who kills with old timey cartoon antics. One of her victory poses has her taking a puff off her cigar and exhales the smoke from the corner of her eye. And speaking of those eyes, those dots on her face were her eyes, and she actually sees with the eyes on her arms.
 * And speaking of eyes, in her Story Mode there's a sequence in which the player is treated, from Peacock's point of view, to a flashback in which one of the slave traders reaches his thumbs towards her eyes. It ends with the screen going red and a sickening squelch.
 * Though she seems to be content with her new lot in life, unlike Painwheel. Though that might just make her scarier.
 * Ms. Fortune. Her attacks are extremely graphic compared to the rest of the cast's, with blood and muscle fibers exposed whenever she detaches her limbs, and her backstory has her being dismembered by the Mob shortly after attempting to steal and subsequently eating their treasured Life Gem. It's unknown if she happened to feel any sort of pain during that ordeal and she retains the scars from her dismemberment.
 * Her fate in Cerebella's ending. Graphically crushed in Vice Versa's massive hands until all that's left is the Life Gem that gave her immortality.
 * Painwheel. Like Peacock, she was kidnapped in order to be turned into the killing machine she is today. She's in perpetual torment, so she's mentally controlled by Brain Drain, a girl trapped in her own body. She's like a nightmare that you can never wake up from.
 * And her fighting style makes it so she's constantly contorting her body into positions that would be excruciatingly painful in real life. For example, at least one move involves pulling her leg up to the point it seems to snap and sprouting a huge spike through the back of her knee.
 * Valentine's Level 3 Blockbuster. She stuffs her opponent into a body bag and Izuna Drops her onto an operating table. The screen goes into a cinematic with the victim looking up at her through dim operating lights. And then...

"Peter Bartholow, Reverge Labs: After we submitted the first drafts of the stories, our producer at Autumn sent us an e-mail that basically said "...Is Alex feeling OK?" But, yes, these are all different ways that things go "wrong," despite having a number of overlaps with the "real" events."
 * There's also the fact that she leaves the entire operating curtains splattered with her victim's blood. Just what the hell did Valentine do to her victim to spill that much blood in a blink of an eye?!
 * She also has one particular Story Mode portrait that's a bit creepy. The one where she clenches her mask between her teeth in a Slasher Smile.
 * Double is terror incarnate.
 * Double's Story Mode portraits is more disturbing than her usual appearance.
 * Her Story Mode ending shows
 * The non-canon Multiple Endings in the game's initial story mode.