Samurai Jack/Tear Jerker


 * Near the end of episode L (#40), when X9,  Cue Jack showing a worried expression at possibly killing a perfectly innocent robot.
 * "Lulu... sweet thing."
 * How can you forget "Jack and the Lava Monster"? Jack fights his way through a maze of death only to confront a near-invincible giant lava monster Then we learn that  Blast it, I'm tearing up just writing this one.
 * "Free"
 * The ending to "Jack and the Mountain."
 * "Jack Remembers the Past" is heartbreaking.
 * The season five premiere reveals that thanks to Aku, Jack can no longer age. Sure, he can die, but we see that eternal youth is a curse for him. He can definitely develop facial hair, and he is mentally in his sixties given the cynical look when he shows up on a motorcycle and saves a family..
 * Jack has a My God, What Have I Done? reaction when he sees that the Daughters of Aku are human, after slitting one's throat in self-defense. It takes him an episode to remember that sometimes you don't have a choice but to take a human life, especially when you can't reason with a person wielding a deadly weapon.
 * Ashi eventually comes to the horrible Heel Realization that not only is Jack definitely not evil, but that she was raised as a weapon, for a big lie. She believes him when he shows her the factory where children are enslaved, and goes to save him when he goes on a suicide run.
 * The fight between Ashi and her mother. It is awesome, as Ashi defends Jack and calls out the Priestess for stealing her childhood, but also sad that Ashi acknowledges that her sisters died for nothing, and it wasn't Jack's fault. Jack was defending himself against a hostile threat, and Ashi needed a long time to listen to him.
 * The fact that in the finale,.
 * For the series as a whole, especially regarding the ending: