Big No/Western Animation

"Patrick: Maybe it's just because you're ugly.
 * Used in the Teen Titans episode "Birthmark" by Raven when Slade shows her a vision of a destroyed world that she's going to create. Also used by Raven in the same episode when she refuses for a final time the party the others have planned for her birthday and destroys everything with her powers.
 * The Penguins of Madagascar are in love with this trope. Most come from Skipper.
 * SpongeBob SquarePants:

SpongeBob: Ugly?! (sticks finger in mouth, slides it across his eyebrows) You gotta be kiddin' meh.

Patrick: Let us try the reflection test. (holds up a mirror in front of SpongeBob)

SpongeBob: (to reflection) Hi! (SpongeBob's reflection grabs a hammer and breaks itself )

Patrick: Uuuuuuglyyyyyyy....

SpongeBob: OH NOOOOOOOOOOOOOOOO!!!!!!!"

"(Homer gives a running dive with an unenthusiastic Big No)
 * Also used in "Bubble Buddy", in dramatic slow-motion and with added vowel screwing around with.
 * Several times throughout King of the Hill. Nearly always by Hank. One of the funniest parts of the show.
 * Sev Trek: Puss in Boots (the Australian CGI spoof of Star Trek the Next Generation). Captain Pinchhard utters one of these, upon hearing that Measly Cruncher is going to save the Enterforaprize for the 47th time.
 * The Simpsons
 * In the episode "Mayored to the Mob", bodyguard-in-training Homer practices his slow-motion dive. Making it funnier, the instructor is voiced by Mark Hamill, who should know his Nooooos...

Instructor: Well, your lunge was good, but I just couldn't believe your 'NOOOOOO'! You gotta sell it! Remember, your 'NOOOOOO' is what gets you paid! Now drop and give me 20!

Homer: NNNNOOOOOOOOOOOO!

Instructor: Better."

"Barney: (after noticing the others have normal eggs and slowly seeing he got the black one) NOOOOOO! (crushes the egg in his hand and holds his head)
 * Used straight in episode "The City of New York vs. Homer Simpson", when Barney discovers he holds the black pickled egg that means he's today's designated driver.

Homer: (coming up to him) You got the black one."

"Ned: Do you reject Satan, and all his empty promises?
 * Also used straight by Bart in "Black Widower".
 * Seymour Skinner does three in a row in the episode "Bart's Comet", each with increased intensity; firstly, when he discovers that Bart has managed to discover a comet, something Skinner has dreamed of for years, when he briefly left Bart in charge of his telescope whilst trying to capture a less-than-complementary hot-air-balloon depicting Skinner with a giant rear that Bart earlier released ("Noooo!"). Then, he realises that in his earlier grief he's released the hot-air-balloon, letting it float free to humiliate him further ("Noooo!"). Then, a paperboy drops the morning paper in front of him, with a headline reading "PREZ SEZ: SCHOOL IS FOR LOSERS": ("NOOOOOOOOOOOO!").
 * Skinner pulls a similiar one season 4's "Duffless".
 * Done yet again in "Homer and Lisa Exchange Cross Words". Homer makes Lisa so angry that she tells him that she's going to use Marge's maiden name (Bouvier) instead of Simpson. Homer drops to his knees and screams "NOOOOOOOOOOO!" Then another girl walks buy and asks if he wants to buy some Band Candy. Homer's reply: "YEEEEEEEEEEEES!"
 * And then there's the episode where the Flanders's get Bart, Lisa, and Maggie as foster children.

Homer: NOOOOOOOOOOOOOOO!!!!!!! (skin burns on contact with holy water)"

"Lisa: Dad, no! [The poisoned eclair] is going to kill you!
 * "Guess Who's Coming to Criticize Dinner?":

Homer: Eh, I've had a good run. (prepares to take a bite)

Lisa: No! (thinks) It's low-fat!

Homer: NNNNNNNNNNNNNNNOOOOOOOOOOOOOOOOOOO! (throws eclair)"

"Grandpa: NOOOOOOOOOOOOOOOOO-- (cut to show that he's headed for a stack of lawn gnomes) --OOOOOOMES!"
 * And parodied yet again in an episode where Grandpa is a Sprawl-Mart greeter, and gets knocked onto an out-of-control shopping buggy.

"Policeman 1: Are you okay?
 * In "Homer's Barbershop Quartet", Chief Wiggum lets out one that gets twisted into a wolf-type howl at the moon.
 * In the beginning of the episode where Bart made a new friend who was the daughter of Wolfcastle, Marge threw the Olympic Torch to the police and the police helicopter crashed and...

Policeman 2: Yeah, im ok...(looks at extinguished Olympic Torch)"NNNNNNNNNOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!!!!!!!!!!""

"(Homer bursts in)
 * In the "138th Episode Spectacular", alternate clips are shown including another ending as to who shot Mr. Burns. When he fingers Smithers as the culprit, Smithers gives a big no then adds, "Wait a minute. Yes."
 * Played for Laughs in the episode where Marge gets a job at the Power Plant. Smithers is finished cleaning the unirals until...

Homer: Oh, man! I really gotta-- (unzips fly)

Smithers: NOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!!"

"Dr. Zoidberg: (in the middle of eating and nonchalantly) I'm a doctor. He's dead.
 * Many in Futurama. In particular, being a parody of melodramatic acting, Calculon the robotic soap-opera star is prone to do this sort of thing a lot.
 * "Bend Her" has two in one episode. Calculon is being interviewed on a talk show with Coilette as another guest (who is Bender who changed his robotic sex and invented his own country to win multiple fembot Olympic medals). When asked to set the audience up for the clip they are about to show from a new episode, Calculon responds "No, I think it's self-explanatory." The clip shows Calculon screaming "NNNNOOOOOOOOOOOO!" (while a Pirate inexplicably cooks burgers on a barbecue behind him). After the clip, Calculon nonchalantly replies, "Funny story: The script called for me to say 'yes' but I gave it a little twist." Afterwards, "Coilette" ends up engaged to Calculon but has to call the wedding off before the sex change becomes permanent. After... she fakes her death of at what would have been her wedding to Calculon...

Calculon: No! NNNNOOOOOOOOOOOO! N! O! O! O!"

"Professor: No! Noooooo! But possibly yes."
 * Done again by Calculon in "That's Lobstertainment!", when Harold Zoid (Zoidberg's over-the-hill celebrity uncle) is trying to make a movie that will guarantee him an Oscar.
 * In "Mars University", Gunther the genius monkey decides to get a degree in business with his now only decent intelligence. causing Professor Farnsworth to shout a big no.
 * In "Love and Rocket", Bender dumps the crazy Planet Express ship while she, and the main cast inside her, are being chased by a large fleet from Omicron Persei 8. Cue big, shrieking no.
 * "When Aliens Attack": Fry gets one off after a ship from Omicron Persei 8 blows up his sand castle, immediately after the Omnicronians blew up many world monuments gathered in the same part of New New York.
 * And Fry again in Into the Wild Green Yonder when Bender gives him back his game (he was playing Tetris) and Bender tell him he missed up the "L" formation he was making with the blocks.
 * Fry again in "Fry and the Slurm Factory", when Leela dumps the trough of pure Slurm he'd been eating from into the sewers.
 * Fry again in the episode "Time Keeps On Slippin'" when his love letter to Leela ends up being destroyed.
 * Fry yet again in "Benderama" when the "disfigured" giant,
 * Bender in the episode Lethal Inspection when
 * In "Benderama", during an episode of the Show Within a Show "The Scary Door". A scientist builds a robot that does everything for him, including shouting "NOOOO!" when the irony of the Karmic Twist Ending sinks in.
 * In "The Duh-Vinci Code" when Fry accidentally destroys The Professor's Beard of Leonardo Da Vinchi only for him to find a hidden scroll in it a second later.

"Singer: But Megabyte betrayed Bob and
 * Played with by Bender in "Silence of the Clamps" where, after delivering a Big No as he, describes the scene in hilariously full detail in front of the chief of police, complete with imitation of said Big No. When required to testify before a grand jury, he
 * In a Robot Chicken sketch involving Archie and his friends dealing with deaths plaguing everyone, Moose lets out a Big Duh when Ms. Grundy is crushed by a car.
 * In the Star Wars Robot Chicken Special (3rd one), Vader slips on something, and falls. His hand gets stuck in the toilet, and the toilet flushes. Cue the: "NOOOOOOOOOOOO!"
 * Jackie Chan Adventures:
 * Shendu yells this after ending up possessing Valmont, instead of Jackie, which was what he intended. Almost all of the Demon Sorcerers scream this when they are banished back to the Netherworld through their portals. When they banish the Earth Demon, Jade hangs a lantern on it: "Here comes my favourite part. "NOOO!""
 * Uncle is also fond of the Chinese version "Iyaaaa!" -- usually just before smacking Jackie around the head for doing something stupid.
 * Parodied throughout the Drawn Together episode "Terms of Endearment", in which Captain Hero screams "Nnnooooooo!!" at least 7 or 8 times. At one point (as the camera pulls back... and back... and then back some more, to reveal just how big a problem he's facing) Hero screams the Big No three times in a row, with dramatic pauses in between.
 * Often used by Swat Kats villains such as Dark Kat and The Pastmaster when the heroes defeat them.
 * Done straight on a fairly regular basis in Re Boot. . This YTMND does a rather good job of pointing out just how often this trope is played.
 * Lampshaded in the musical recap at the end of the third season:

He threw him deep inside the pit.

The pit was closed,

and Bob was hosed,

and all that he could say was-

Actor Bob: Noooo!"

"Nick Ibsen: You are all going to be reunited with your families.
 * The X-Men animated series:
 * A wonderful Big No can be found in episode 4 of season 2, "One Man's Worth part 1". The first occurrence is when Bishop shouts it as Professor X, followed quickly by an epic Big No from Prof. X when . The episode ends with a repeat of the opening scene -- but this time, it's in slow motion for the last few lines of dialogue, including Bishop's Big No. Behold.
 * Also there's a Big No from Apocalypse at the end of the four part "Beyond Good and Evil".
 * As well as one from Wolverine in "Out of the Past: Part 2" when Yuriko was touched by the M'Kraan Spirit Drinker.
 * In addition, there's one from Cyclops in "Reunion, Part 1" when Sinister and his gang kidnaps Jean.
 * Another Big No scene sadly fails in X-Men Evolution, when Nightcrawler reacts too slow to stop, shortly after finding out that Mystique was his mother. Again, the scene would have been so much better without the slow-motion yell. When recapping that episode in the introductions to later ones, they put the "NOOOO" back at normal speed. It's much more effective.
 * Transformers
 * Optimus Primal gets quite a few in Beast Wars, most notably in the cliffhanger of the first season finale, and when  in season 3.
 * Inverted with Megatron, as he usually says "Yesssssssssssssss."
 * Last episode of Beast Machines. Megatron's final word? "NOOOOOOOOOOOOO!"
 * And in Armada, Optimus Prime gets one of these when.
 * Parodied in the "Bee in the City" script reading of Transformers Animated, with David Kaye using his Beast Wars Megatron voice (to enthusiastic approval from the crowd). Upon his defeat, he vows to return, ending with a Big Yes "YEEESSS!"
 * In Animated, the Autobots discuss having to leave, and Bulkhead wonders how Sari (human sidekick) will take it. Bumblebee says she's a big girl and can handle it. Cut to Sari screaming NOOOOOOOOOOOOOOOOOOOOOOOOOOOO!! at the top of her lungs.
 * Not to mention the episode "Nature Calling" when Prowl smashes Bumblebee's only battery, rendering him unable to see movies in the middle of nature. His "NOOOO!" was truly epic.
 * In The Spectacular Spider-Man, the Sandman gets one of these after seeing the results of his Transformation Trauma into a living sand being.
 * Littlefoot gets one of these in The Land Before Time IX: Journey to Big Water, as he watches Mo lead the swimming sharptooth away.
 * Used in American Dad by either Stan, Francine or Hayley when . Rather effective due to how strangled and broken it sounded. Steve also gets a particularly good one.
 * Avatar: The Last Airbender
 * There's a slow-motion one from in the Grand Finale when . Cue "must run in the family" jokes...
 * It really does; Iroh has a (much less dramatic) one when he sees June get hit with her Shirshu's paralyzing tongue.
 * There's also one in the desert episode, right before Aang creates a giant mushroom cloud.
 * Parodied in the Who Would Want to Watch Us?}}? episode when Actress Aang lets out a nice 9 second "NOOOOOOOOOOOOOOOO!".
 * Katara has one in the Puppetmaster when.
 * Danny Phantom
 * When Danny's family and friends (and teacher) are about to be blown up to their doom in an alternate timeline, Danny cries one out, complete with slow motion movement.
 * Vlad later gets one in another episode when his perfect clone is destroyed by Danny and his Opposite Sex Clone. For just one word, it's brilliantly acted.
 * In one episode of the 2003 Teenage Mutant Ninja Turtles 2003 series, Baxter Stockman lets out a big "WHY?" after Bishop brought him back from the brink of death (as a result of the events in "Insane in the Membrane"), which robbed him of finally resting in peace after everything he lived through in the series. It...comes across a bit narm-y.
 * Kim Possible
 * In "Stop Team Go", after using hundreds of mooks to destroy the gurken jar, Dr. Drakken puts the jar under a big laser beam, and he fires it. The result was a big crater on the floor, and the jar was unharmed. Cue Big No.
 * In another episode, upon learning that her very annoying younger twins have been skipped ahead and are now in the same high-school as her, Kim lets out a big no powerful enough to knock letters of the school sign out front, while she's indoors.
 * The Powerpuff Girls
 * "Daylight Savings": "PARENT-TEACHER CONFERENCE?! NOOOOOOOOOOOOOOOOOOOOOOOOOOO!"
 * Captain Righteous and Lefty confront the Ministry of Pain in "Fallen Arches". The Captain lets out a big no as he runs towards Lefty who just hurt his hip. However, he hasn't reached his sidekick yet so he shouts it again.
 * Parodied in the Space Ghost Coast to Coast episode "Boo Boo Kitty", when Space Ghost's pet cat, actually a large balloon, slowly drifts towards a large safety pin. He screams NOOOOOOOOOOOOOO!, runs out of breath, inhales, and starts again. After the balloon deflates, he promptly ignores the others when they suggest he could have easily saved Boo Boo Kitty from its untimely death.
 * Played for laughs in Metalocalypse, where Big Nos are Nathan's catchphrase, and he lets them out quite regularly. They are usually accompanied by the camera flying down Nathan's throat. One example comes when Nathan lets out a NOOOOOOOOOO! upon Nick Ibsen revealing Dethklok's parents.

(a panel opens up, revealing the families of each of the five band members)

Nathan Explosion: NOOOOOOOOOOOOOOOOOOOOO!!! You're going to pay for this!

Nick Ibsen: Pay for what? Journalistic integrity?"

"Freakazoid: NO! A big N, followed by a little O: no!"
 * The bit on flying down the throat comes from the episode "Murdering Outside the Box". When Nathan learns that someone is embezzling from the band, he suddenly goes into the Big No sequence, and from the "down the throat" bit the show goes into the opening.
 * It also happens when Nathan, after having death-growled the entire works of Shakespeare to sell as an audiobook, learns that an extremely-inebriated Pickles "like...TOTALLY forgot to hit record, man." The strain of recording the growled version of "Hamlet" having taken its toll on Nathan's larynx, the Big No was rasped rather than screamed.
 * The Buzz Lightyear of Star Command episode "Plasma Monster" has Booster react this way when Plasma Boy's girlfriend tells him they can still be Just Friends.
 * Ace Lightning: The characters in this mixed media show are based on superhero stereotypes, so this was going to show up sooner or later -- except that it's not one of the CGI characters that do it -- it's a human, in the last episode, when he thinks Ace has been shot.
 * In Street Fighter, Guile gives one when Cammy defected to Shadaloo and gives Bison a big kiss. Note the Soundtrack Dissonance.
 * In Freakazoid episode "House of Freakazoid", Freakazoid is pressured to pursue a villainess through a sewer, but repeatedly refuses because he hates the smell of poo gas.

"Klump: General Klump reporting, King K. Rool sir! Y'read me, your high 'n mightyness?... Looking a little pale there, chief!
 * Parodied in The Fairly Odd Parents "Wishology: The Exciting Middle Part", which ends with a big "Dagnabit!"
 * Played straight in the Fairly Oddparents: Shadow Showdown video game; Timmy shouts it after his TV breaks in the introduction, and again upon seeing he's missed the Crash Nebula season finale at the end.
 * And parodied in "Pixies Inc", where, after being foiled, the Pixies let out a big no--with no emotion.
 * Cow of Cow and Chicken did it in an episode while jumping to catch a coin. In slo-mo. With the entire world slowed down as well.
 * In the last episode of the third season of The Venture Brothers, Henchman 21 indulges in a Big No when the Monarchmobile explodes, but is interrupted when   hits him in the chest.
 * In Brother Bear, Kenai lets out the distant, bird-disturbing version of a Big No when he discovers he's been transformed into a bear.
 * In Batman Beyond, Derek Powers accidentally inhales a big gulp of his own nerve gas, and in the same second it takes him to realize what has happened to him, makes an appropriately horrified scream to this effect.
 * Shriek also does this once he realizes his own sound amplification device has rendered him deaf.
 * An episode of Family Guy has Peter be really upset over the loss of his pet parrot.
 * The Donkey Kong Country episode "Buried Treasure" contains this exchange when Klump's video conference interrupts K. Rool's computer game:

K. Rool: NNNNNNNNNNOOOOOOOOOO!!!!!!!!!!!!!!!!!!! (smashes the computer)

Klump: (calling on the phone) Chief? You there, chief? Seems we had some sort of interference."

"Kyle: (long speech) I don't want a fighter. I want my mom.
 * In an episode of Flap Jack, The Real Adventurer has the reaction to Bubbie telling him K'Nuckles is not a pregnant woman.
 * Invader Zim just loves to scream. The Big No is his favourite.
 * From the same show we also have the most epic Big No of all time as delivered by a random guy at a park bench who's soda can is being crushed by Mars.
 * And then there's two Big No's in the episode "Zim Eats Waffles".
 * South Park:
 * The episode "Jewbilie" does it four times in a row: First Kyle when Kenny makes a Heroic Sacrifice to stop Haman, then Haman when Moses escapes the conch shell, then anti-semetic Rabbi Garth screams it twice when he realizes his plans have been foiled.
 * The Movie:

Soldier: (moved, sniffs) Poor little fella.

Sheila: NOOOOOOOOO! (wrestles rifle out of the general's hands and shoots Terrance and Phillip)"

"Mormon: We were just about to do a play about how much stealing hurts you deep inside. Come join us. (the Mormons lift Saddam over their heads and carry him off)
 * "Probably": God gives Saddam Hussein an even worse punishment than sending him to Hell: sending him to Heaven.

Saddam: No! NOOOOOO!"

"Stan: Why can't I be in a boy band?
 * "Something You Can Do With Your Finger" parodies the Star Trek First Contact example above, when Randy prevents Stan from being in his boy band. Incidentally, they actually use the sound clip of Picard's breakdown from the movie for that scene.

Randy: (firmly) Because I said so.

Stan: Cartman said we're going to perform at the mall at three o'clock! My friends are gonna be pissed off at me!

Randy: (still pretty controlled) Let them be pissed off.

Stan: Dad, I don't understand. Just let me do this one thing and then I'll...

Randy: (inexplicably losing it) Noooo! NOOOOOOO! (smashes a glass dresser with his head)"

"Daffy Duck: All right. Let's get this picture started.
 * In "The Last of the Meheecans", Randy does it again when he's confronted with what happens when Mexican immigrants performing menial labor all decide to move back to their birth country in a fit of nostalgia, using the same Darth Vader soundclip from Revenge of the Sith.
 * In "Marjorine" Linda Stotch lets out several blood-curling screams of "NO" when she thinks her son Butters has committed suicide by jumping off a building. She does it again at his funeral while throwing herself on his casket.
 * Rocko's Modern Life: Rocko and Heffer are on a bus tour of Paris with a bus driver who is determined to keep his passengers on the bus at all costs, so much so that he chases them around the city after they escape. By the time the two board a plane home, the bus driver delivers an epic BIG NO as the two fly off into the sunset.
 * Spider-Man the Animated Series: Blade lets out a particularly narmtastic one of these in the season four episode, The Vampire Queen. And Spider-Man gives a particularly haunting one upon.
 * Total Drama Island
 * Owen says a big no when a box of chocolates gets knocked into the water.
 * Also, Alejandro in the finale of the 3rd season gives out a Big No after he is . Al lets it out when he asks Chris if the prize money is safe, only to find out . It's hilarious because it parodies the cheesiest moments in Star Wars history (which is seen in the picture above).
 * Phineas and Ferb
 * In "Gaming the System", Candace does this in response to her brothers' Disney Death. A few minutes later, Phineas yells one when the game's final boss goes after Isabella.
 * In "The Chronicles of Meap", Mitch apparently lets off a Big No when Meap confiscates his Universal Moustache Translator, but it comes out as a Big Meap.
 * Candace lets out another Big No in "Invasion of the Ferb Snatchers".
 * Parodied in "Lawn Gnome Beach Party of Terror" the radio DJ at first lets out what appears to be a big no... but he's actually screaming "Gnomes"!
 * Parodied in the Musical Episode of Daria. Daria gives a Big No as Kevin accidentally locks the four of them out on the roof.
 * In one episode of Adventures of Sonic the Hedgehog, Robotnik lets out a Big No which is used extremely often in Youtube Poop. This video is also an Overly Long Gag.
 * Dante's Inferno: An Animated Epic - Dante lets out a pained and enraged (and kinda narmy) big no as he beats on the Gates of Hell that separate him and his wife's soul.
 * This has happened more than once in Winx Club.
 * Mung Daal lets one out during an episode of Chowder.
 * Batman the Animated Series
 * Episodes "Pretty Poison" and "Eternal Youth", each near the end, have the villainess Poison Ivy saying this trope.
 * The episode "His Silicon Soul", near the end, has the cyborg Batman saying it after.
 * Used in the episode "Mad Love" by Harleen Quinzel after The Joker gets captured again and taken away from her which helps to show us her Sanity Slippage.
 * A few Looney Tunes/Merrie Melodies cartoons have this:
 * The Chuck Jones Warner Brothers cartoon Duck Amuck:

(Iris Out and THE END appears)

Daffy: Nooooo! Nooooo!"


 * The Friz Freleng cartoon Bucaneer Bunny has Yosemite Sam (a.k.a. Pirate Sam) say a couple of Big No's when Bugs attempts to throw a matchstick inside his pirate ship which is filled with gunpowder.
 * Also one near the end of the McKimson short "Sleepy Time Possum" in which Paw Possum, disguised as a dog, gets catapulted by his son.
 * The Bonkers episode "In the Bag" has Lucky Piquel say this trope when both Bonkers and Lucky are chasing the bag in his police car only to find that the brakes are missing.
 * Jimmy Two-Shoes: Beezy lets out one as Heloise beats him in a staring eyes competition.
 * Eek the Cat lets one out in the episode "Cape Fur", when he's being dragged off by JB for punishment after he hears about the Killer Rabbit on the news.
 * Happy Tree Friends, while not exactly a "No!", has the very drawn-out "Yarr!" in the episode Sea What I Found. Given the circumstances, it's obvious that he means no.
 * In The Adventures of Jimmy Neutron Jimmy gets one when he travels to the future and he discovers that his future self married Cindy. And when it comes back from commercial, we discover that he screamed for four minutes.
 * Sheen lets out one in the interstitial shorts after his Ultralord action figure gets stuck in a tree. Also lets one out in Ultra Sheen when he witnesses Ultra Lord's defeat at the hands of Robo-Fiend inside the the video game
 * Hugh does this in "Attack of the Twonkies" when Judy takes Flippy from him and throws him to Jimmy so he can power up his rocket and send the giant Twonkie back into space.
 * A Tom and Jerry short revolved around a duckling imprinting on Tom and Tom taking advantage of this to attempt to cook the duckling. Near the end, the duckling realizes that Tom intends to eat him and prepares to take a long walk off a soup spoon into a bubbling pot, "If my nice mommy wants a duck dinner..." Tom utters a Big No as he catches the duckling. A "No!" made all the more impressive by the fact that Tom & Jerry usually don't have speaking voices....
 * This could easily double as a Crowning Moment of Heartwarming
 * Happens in an episode of Nightmare Ned by the Tooth Fairy when Ned wins a game of checkers against a giant tooth for ownership of his teeth.
 * Little Dog in the Two Stupid Dogs episode "Cat!" after Big Dog goes back to sleep without scaring the cat.
 * This is Johnny Bravo's reaction to finding out the Easter Bunny isn't real.
 * Wakfu
 * Adamaï lets out a Big No (with Skyward Scream) at the end of episode 16, after Grougaloragran teleports the heroes away to fight Nox alone.
 * Nox, as part of his Villainous Breakdown in the season 1 finale, when.
 * Happens quite often in Dexters Laboratory. One time in particular, when Mandark forces Dexter to shut down his laboratory.
 * The Death Kwon Do instructor in Regular Show gets three rather nice ones. First when he discovers Rigby stole the instructions to learn the Death Punch of Death, only to be interrupted by a student explaining the toilet was 'Death Kwon clogged', leading to another Big No. Then finally, when he discovers that Mordicai stole the Death Jump and Death Dump instructions, he lets out another Big No.
 * Scaredy Bat lets one out in an episode of Ruby Gloom.
 * Spike does this twice in the My Little Pony Friendship Is Magic episode "A Dog and Pony Show", both times coupled with Skyward Scream: the first when Rarity is kidnapped, and another when he realizes he and the other ponies have gotten themselves lost in the Diamond Dogs' mine.
 * Nightmare Moon does it in "Friendship is Magic, Part 2" when the Mane Six activate the Elements of Harmony and blast her with them.
 * Likewise, Discord does it in "Return of Harmony, Part 2", when the Mane Six use the Elements to turn him back into stone.
 * It seems to be tradition for the big baddies to give one upon defeat, as the Big Bad of the second Season Finale, lets off a very nice one just before  Her Voice of the Legion makes it especially nice.
 * In "Stare Master", Fluttershy's reaction to Applebloom suggesting the Cutie Mark Crusaders try to get coal-mining cutie marks is a very emphatic (especially for her) "NO!"
 * Scootaloo does this in "Cutie Mark Chronicles", when Fluttershy suggests singing another (cutesy) song.
 * The Cutie Mark Crusaders do this at the end of "Hearts and Hooves Day" when
 * In Veggie Tales, Larry the Cucumber did this when Archibald inserted "His Cheeseburger" in the jukebox. "NOOOOOOOOOOOOOOO..."
 * Johnny delivers one in Johnny Test. And then the ground shakes.
 * Richard in The Amazing World of Gumball, when told he has to get a job.
 * Princess Sally yells it in Sonic's bad dream when she is roboticized in Sonic the Hedgehog, episode "Sonic's Nightmare".
 * Utterd by Kabuto at the end of Tokyo Mater as a result of him losing to Mater in a drift race and is about to have all of his modifications pulled off his body by all of the other cars in Tokyo, who then laugh at him when Kabuto is finally naked.
 * Recess uses this a number of times, a few being "No...no...NOOOOOOO!!!!!":
 * "The Break In": Towards the end, by T.J. when he just broke himself out of detention and onto the playground, just as recess ended for the day.
 * "The New Kid": By Gus when his name is taken away and must be refered to as "New Kid"
 * "The Experiment": By T.J. and Spinelli when they find out they have to kiss each other to see what the effects were on the opposite sex.
 * "The Legend of the Big Kid": By Vince when he and the gang are looking for T.J.
 * "Mama's Girl": By Spinelli when no one will stop taunting her after she accidentaly called Miss Grotke "mama".
 * In Thundercats 2011 Lion-O performs one that overlaps with a Slow No and a Futile Hand Reach upon seeing his father King Claudus stabbed In the Back.
 * Green Lantern pulls off a gloriously hammy one when he's trapped inside a yellow smiley face time bomb in DC Super Friends.
 * My Life As a Teenage Robot: Vexus at the end of Escape From Cluster Prime, when Vega and her friends give Cluster Prime back their golden chips. Also, used in "Puppet Bride" by Mrs. Wakeman (along with a flying tackle) during a wedding between Little Acorn and Jenny.
 * Happens quite a lot on Adventure Time. In one episode, the Kid Hero Finn has to win a contest in order to win a kiss from his love interest, Princess Bubblegum. When one of the other contestants almost wins, he lets out a big no that reaches the level of a Sonic Scream.
 * Also lets one out in Mystery Train when Finn sees that.
 * Aqua Teen Hunger Force: Meatwad gives one when he finds out Shake ate Inside-Out Boy.
 * Transformers Prime has Knock Out doing one when Starscream punishes him in "Speed Metal".
 * Miko does one when Bulkhead almost gets beheaded in "I.M.I.".
 * Arcee yells out a "No!" when
 * Megatron gets a magnificent one during "Orion Pax Part 3" when
 * The Ren and Stimpy Show: In "Sven Hoek" Ren lets out a Big No after he sees that Sven and Stimpy ruined his collection of rare incurable diseases.