Spider-Man: The Animated Series/Funny

"Kingpin: Spider-Man. Having you here is a real treat. Spider-Man: If I were you, I'd cut down on the treats."
 * This exchange between Kingpin and Spidey after he's capture by the Six.

""Power of Lightning, strike again!"
 * "You want a trial separation already. I understand but... I'm hurt."
 * From the Crossover with X-Men:

"Um... Power of Webshooters, get real sticky!""

"Security Guard: Look, Spider-Man really is the thief! Spider-Man: Oh, great. Jameson will probably be selling 'Spider-Man really is the thief" t-shirts by noon tomorrow."
 * "A nuclear reactor? Why don't villains ever pick bakeries to invade?"
 * "It's not nice to rob jewelry stores in Mister Spider-Man's neighborhood. Can you say, 'I'm going to jail'?"
 * "Rhino and Shocker? How'd they get together? Computer dating?"
 * "SHOCKEEEER! YOU CAN'T ESCAPE ME! I'LL CHASE YOU TO THE ENDS OF THE EARTH!" It's his over-the-top delivery that really sells it.
 * From Hydro-Man:

"Alicia: "Well, at least I won't be changing diapers anymore..."
 * "I wouldn't be so sure!""

""Only problem here is, I don't know who to root for.""
 * I don't know about anyone else, but this Troper dies laughing every time baby!Silvermane ever opens his mouth. Just hearing a little kid's voice saying things like "Bah! My mind is trapped!" is hilarious.
 * Tombstone observing the fight between Spider-Man and Smythe's Mega-Slayer:

"Curt Conners: You weren't born with those powers, were you? Spider-Man: No, I won 'em on a game show."
 * An alternate reality's Gwen Stacy explaining that she recognized Spider-Carnage as the impostor because he's "as nutty as a fruitcake."
 * Or Spider-Man: The Animated Series:

"Conners: Amazing. The tests would show Neogenics was involved! But how could that be?
 * Later:

Spidey: That was the category I chose."

"Spider-Man: You had to wreck an entire civilization to prove that evil is bad?"
 * After The Beyonder unleashes a horde of villains on an unsuspecting world for an experiment. Spidey mixing incredulity with sarcasm is just hilarious.