Sayonara, Zetsubou-sensei/Tear Jerker

"However,"
 * Even the normally over-the-top ridiculous Sayonara Zetsubou Sensei has one of these in Episode 11 of the second TV series, when the girls sadly explain what has driven them to murderous insanity. "But... he never chose any of us".
 * The ending of the eleventh episode of Sayonara Zetsubou Sensei is bad enough, as it moves completely away from the usual wacky and over-the-top tone.  And if that isn't tearjerker-y enough for you, here's this AMV/parody ending.