Transformers Animated/Funny

"Blitzwing: "You stepped in something!" *faceswap* "But with a few dancing lessons, you'll soon be as light on your servos as me, cha, cha, cha!""
 * Optimus Prime asks Sari how humans make "these new, smaller organics" (i.e. babies). His reaction to what he hears is priceless.
 * "I am Wreck-Gar! I dare to be stupid!"
 * Newly immortal Starscream's death montage.
 * Just about any scene with Blitzwing and Lugnut in it, but especially their first episode.

"Sentinel [as Optimus fights the Headmaster, who has stolen Sentinel's body]: You're enjoying this, aren't you, Optimus? Optimus: [smirks while continuing to pound the Headmaster, which Sentinel can feel]"
 * There was a scene before that as well. Never has "The Itsy Bitsy Spider" been this funny.
 * "I will pound you into sheet metal!" *faceswap* "Wait, we've already got some!"
 * "Ohhh, you're scared of me! BOOGABOOGA!" "AHH!" The best part is probably Jazz's terrified pose.
 * "You interrupted my SPEEEEEEEEEEEEECH!"
 * How about the entirety of "Return of the Headmaster"'s Sentinel-schadenfreude?


 * In the Allspark Almanac databook, a self-serving diary entry from Sentinel Prime makes this even better. "Let me say that again. Optimus. Ripped. My. Arm. Off."
 * Also, "You kicked me! On PURPOSE!"
 * At the end of the Season 2 finale

"Starscream: This day couldn't possibly get worse!
 * And before that:

[Megatron and Blitzwing appear before him]

Starscream: I stand corrected.

Megatron: Staaaarscreeeeeam!!!

Starscream: Sorry, you must have me confused with some other Harrier jet!"

"Sentinel: *Sees a crashed Starscream* What is that thing?! Optimus: Huh, that's right, you've never seen one up close. It's called a "Decepticon"."
 * And then a little later from the same episode (especially considering that Optimus and Co have been spending several episodes trying to convince the Elite Guard that there are Decepticons on Earth):

"Bumblebee: Maybe we could call Grimlock, or con Lugnut into helping us out!
 * This battle of wills continues in the Season 3 premiere "TransWarped", where Starscream gets so fed up that he starts ranting about how Megatron got them into this mess: "Are you seeing a pattern here? THIS! IS! ALL! YOUR! FAUUUULLLLTTTTT!"
 * Not sure if this counts, but the 2008 Botcon script reading "Bee in the City" is the funniest thing this troper has ever seen/heard, full of shout-outs and fourth wall breaks aplenty. Watch it here.

Joe : Please...who do I look like, Scott McNeil?"

"Optimus Prime: Nnnnnno, we won't be doing that."
 * The entire segment where Shockwave explains what happens when they face a cosmic-powered supervillain, including what the secondary characters do and Optimus Prime's reaction to it.

"Lugnut: STASIS LOCK ITSELF COULD NOT DETER ME FROM YOUR GRAND, AND GLORIOUS PLAN, OH WISE AND NOBLE MEGATRON! Megatron: Just...go...."
 * Vangelus' entire performance as the "morally ambiguous" Transtech Soundwave is brilliant.
 * Megatron giving Lugnut orders in "Garbage In, Garbage Out". Seeing how this is Lugnut....

"Ratchet: Will you get in already!? Sari: Ah, ah, ah, what's the magic word... Ratchet: NOW!"
 * And earlier, Sari trying to teach Ratchet some manners:

"Prowl: I'll stay with Bumblebee. Ratchet: Try to keep him quiet. Prowl: You do realize this is Bumblebee. Ratchet: Oh yeah."
 * After Ratchet saves Bumblebee.

"Ratchet: Personally, I've always found Bumblebee's fake static to be a lot more convincing."
 * Optimus Prime, behind you!
 * Prowl's attempt at Fake Static at the start of "A Fistful of Energon." Jeff Bennett just nails it in making it so awkwardly out-of-place for Prowl, it's Adorkable.

"Prowl: *Facepalming* Not this speech again..."
 * In the first episode, Prowl's reaction to Optimus' 'cog-in-the-Autobot-machine' speech.

"Starscream: Y'see, now [Megatron] even likes Blitzwing better than you. Mmm... *Shockwave meanwhile has walked back onscreen*
 * The rivalry between Lugnut and Shockwave during "Endgame". Special mention goes to near the start of Part 1, when after some jibing from Starscream, Lugnut moves in front of Megatron punting Shockwave offscreen in the progess.

Lugnut: *Slaps Shockwave back offscreen* NO! I am the most faithful!"