Big No/Web Comics

Examples of in  include:

#

 * Bulbasaur, after dropping a little girl in a failed rescue attempt in 151 Hidden Depths.
 * 8-Bit Theater:
 * The aptly titled Episode 400: "'Not Safe for Work, School, or Anywhere You Can Get in Trouble" replaces the Big No with the Big F, or rather the Big "FFFFFFFFFFFFFFFFFFFFFFFF UCK! !".
 * Red Mage uses this as Black Mage is about to burn the Rat's Tail they need for the class change. He puts on a levitation spell so he can do the slo-mo jump (but applies too much and is left hanging for a while), and does the Big No just to be dramatic.

A-E

 * Bob the Angry Flower has it at the end of this strip.
 * In Brawl in the Family, Kirby unleashes one when Meta Knight takes his revenge on him here.
 * Candi presents the rare quixotically hopeful drunken ex-boyfriend version.
 * Captain SNES has had its fair share of them:
 * Dr. Wily does the traditional NOOOOOOOOOOOOOO! When he realises he's in a catch 22 as the VP of Nexus and doing anything to ruin Nexus would cause Blues to torture him, meaning he would have to go against his nature and save it from the stupidity that was President Haggar.
 * Alex Willams, replaces the Big No with the Big FUCK, and purposely extends it to buy time to think.
 * He did a simple Big No before. It lasted something like thirteen comics.
 * Happens in Corner Alley 13 when Noelle notices that her café doesn't have any customers.
 * In Cucumber Quest, Cucumber delivers one as Sir Tomato brings his sword down on him.
 * Dragon Mango spoofs this when Pumpkin's father returns after a four-year journey into faraway lands and gives Mango a rare treat, called a "sweet swirl stick", which Cherry eats in one bite.
 * Darths and Droids has several of these.
 * DMFA has this beauty when a young Abel learns that extra lessons include extra homework.
 * Dominic Deegan also has a big "FUUUUCK!" moment, too. Made all the funnier because it's rare to see him lose his cool like that.
 * Dork Tower audible five miles away
 * Tedd's father uses this in El Goonish Shive, when he's realizes he's said he'll pay for eight teenagers to eat pizza, while at a meeting far away. The person he's talking to thinks her ideas are being criticized. The comic is helpfully titled 'I Graduated from the George Lucas Directing Academy'.
 * Right here in Elven Lacryment.

F-J

 * Used pretty straight in Final Blasphemy here and later in the same issue.
 * Friendship is Dragons, Page 688: Applejack lets forth a Big No after being subjected to an in-universe fanfic filled with cliches, Dead Horse Tropes, and Mare, er, Mary Sue Tropes for hours.
 * Girl Genius has many characters getting a Big No, of course.
 * Gil demonstrates correct form here.
 * Later plays... oddly when Gilgamesh Wulfenbach is led to believe that Agatha was killed by a rogue clank. He begins the wind-up to a Big No before cold logic takes over. However, in a flashback to the same scene, Gil is in the classic "Big No" pose. So it's unclear whether he did one or not.
 * Eotain when Dimo hurled a huge Aetheric Collector Array at her (see the next page - she survived, but lost mount, polearm, vest and some dignity).
 * In The Inexplicable Adventures of Bob when Bob learns his roof is about to be destroyed a third time. It will happen many more times.
 * Riboflavin gets one when he faces a fate worse than death.
 * Irregular Webcomic improved the famous scene from Star Wars. In more than one way.
 * A Nooooo! that bursts out of the panel in Jayden and Crusader.

K-O
"Elan: Hey, did someone in here just drop to their knees and scream, "Noooooo"? I got a feeling...."
 * Cale whips one out in this Looking for Group comic.
 * Loserz parodied the original scene here.
 * ''Ménage à 3': Kiley here.
 * Zii's reaction when Di Di tries to cut her hair into a mullet.
 * Zii here.
 * Kiley again here when she thinks Yuki is going to see her father in Japan when she's in a bad condition.
 * Kiley yet again here.
 * Mulberry
 * Mulberry said one when one comic ended with her
 * She performed another one after Jack showed her a newspaper article detailing the death of Brittany Murphy.
 * From Multiplex, sarcastically, from Kurt.
 * The Order of the Stick
 * Vaarsuvius uses it in strip #504, titled "That's a Surprisingly Common Reaction". Lampshaded in the first panel of the next strip, by Genre Savvy Elan:

"Xykon: No no no no no no NO! [...] Xykon: YES!"
 * Durkon gets one of these in On the Origine of PCs after tasting what is allegedly the "finest beer brewed in all the human lands."
 * And the intermission comic, where... well just see for yourself.
 * V does it again after.
 * Xykon, of all people, does it when his.

P-T
"Professor Utonium: What's that you got there? Is that a... Wh--Th--P...P....
 * Penny Arcade: Gabriel provides this gem upon besting Tycho in a card game.
 * The Platypus Comix story "True Believers" had one after.
 * Powerpuff Girls Doujinshi has the following conversation.

Professor Utonium: NNNNNOOOOOOOOOOOOOOOOOOOOOOOO!!!"


 * Princess Pi
 * Alpha Bitch Sam yells this after getting sentenced to  as punishment for blowing up Piscataway Stadium.
 * Princess Pi herself says this when the new princess, Gertie, makes her genie, Bottle Blonde, encase her own bottle in concrete, so that Pi could not make Bottle Blonde turn Piscataway back to normal.
 * The Princess of Rusty and Co. when she found the White Knight crushed.
 * ''Saturday Morning Breakfast Cereal. Candice didn't appreciate his creative marriage proposal.
 * In Shifters Ben Harkus gives us one of these here after
 * Shortpacked!:
 * This is Robin's reaction to finding out that Felix Gaeta is portrayed by the same man that voiced Nightscream from Transformers: Beast Machines.
 * This is also Amber's reaction to finding out that  It takes her two comics.
 * Silent Hill: Promise: When Vanessa is grabbed into a pit by a...thing.
 * Rhea of Slightly Damned's reaction upon learning that she cannot be reincarnated.
 * Subverted in the third panel of this strip of Sluggy Freelance, where a "Little No" gives the dramatic impact a Big No is supposed to have.
 * Jason from Something*Positive has a fairly unique one when realizing he's just turned down a 3-way with his wife and hot friend.
 * Chris in Sonichu does this when he learns that a Starbucks barista only went out with him as a joke. Apparently, this happened in real life.
 * In Starslip Crisis, Zillion can't even do this right.
 * Today Nothing Happened: This very wiki is responsible in this strip.

U-Z

 * Monica from Wapsi Square had a nice one when
 * Nith from What's Shakin' discovers his home has been destroyed.