Villainous Incest

""Incest is wrong!""

- Uncle Gabby, The Drinky Crow Show

So you have a character who is a villain. He's kicked puppies, tortured defenseless kittens, and done everything he can to make sure he has thoroughly crossed the Moral Event Horizon. What else can you do to show the audience that he is absolutely evil in every way?

You have him engage in certain taboo or questionable activities which are frowned upon by most cultures. Sure, Even Bad Men Love Their Mamas, but some bad men love their mamas a little too much, and this adds to the audience's view of the villain as a disgusting, depraved individual who commits evil and unnatural acts on a daily basis.

Compare Brother-Sister Incest, Parental Incest, Good People Have Good Sex.

Contrast Surprise Incest for when the people involved may or may not know they are related, although these kinds of villains usually don't care. Can cross over with Freudian Excuse if they have a weird relationship with their parents.

Anime and Manga

 * Demon sisters Scanty and Kneesocks of Panty and Stocking With Garterbelt apparently enjoy partaking in strange sexual play involving caviar, figs and goat milk when the mayor isn't looking.
 * Akio regularly has sex with his sister Anthy in Revolutionary Girl Utena. It's both an example of this trope, and an allusion to their nature—incest is rather common among many cultures' gods.
 * may do the same during their car ride, and  very nearly do; at best, it's still a rather blunt metaphor each girl's obsessive or weird relationship with men.
 * Alexis and his sister Augusta in Count Cain.
 * Auguste Beau of Kaze to Ki no Uta uses sex to control and manipulate.
 * Sorath and Tiriel from Shakugan no Shana, squicking the hell out of Shana in the process.
 * The creepy twins Yui Fan and Yui Lan from Full Metal Panic!: The Second Raid.
 * And lets not forget Dr. Hagen and his sister Nina in Agent Aika. Interestingly, she was the creepier one in that pairing.
 * Hansel and Gretel from Black Lagoon (it's with each other...and they're kids).
 * The Black Beauty Sisters in Mermaid Melody Pichi Pichi Pitch are almost certainly a couple (they're the page image for Hide Your Lesbians), but it's never made clear whether they're actually sisters or not.
 * The Long twins from Twilight Of The Dark Master. Taken to a whole new level of creepy (or hilarious) when you find out that their voice actors in the dub are husband and wife.
 * Played for laughs in the Black Butler Hamlet OVA, when Grell/Ophelia gets the hots for Agni/Polonius. Agni thinks it's disgusting, even if he's not related to Grell.

Comic Books

 * An obscure Marvel Universe character from the equally obscure Comet Man series knowingly started dating his estranged and unwitting sister as part of a plan to revenge himself on his entire family. While sex was never made explicit, they had moved in together for some weeks before his relationship to her was revealed.
 * Andrea and Andreas Strucker, the Fenris twins of Marvel Comics. And then It Got Worse squickier after Andrea died.
 * Technically their incest was never confirmed, and Andreas denied it at one point (Well that's not surprising). But the implications are incredibly strong.
 * Baron Mordo, archenemy of Doctor Strange, knowingly had a sexual relationship with his his Gypsy half-niece (she was unaware of the relationship) which ultimately resulted in a daughter. Just to make it worse, Mordo seduced her to steal the Book of Cagliostro, a book of spells, and then abandoned her.
 * While it was implied throughout the franchise that the family may have been committing incest, the comic The Texas Chainsaw Massacre: Raising Cain outright stated it. After one family member's wife is killed (in part, by him) he nonchalantly states he has plenty of other sisters he can marry, also indicating he and the rest of the family are going to eat the dead one ("Meat should never go to waste!")
 * Quicksilver and Scarlet Witch are in a long term relationship with each other in the Ultimate Marvel universe, possibly based on an earlier Memetic Mutation originating in the original comic universe. So much so to the point where Pietro actually gets jealous when Wanda hits on a robot.
 * Although the two of them are more Anti-Hero with Anti-Villain moments as opposed to out and out villains.
 * In Alan Moore's Swamp Thing, Abby is unknowingly seduced by her own uncle, the uberevil villain Dr. Anton Arcane.
 * Strangers in Paradise has Darcy and, yes,
 * Many of the villains in Sandman Mystery Theatre are incestuous in one way or another, to the point where you start to wonder about the writer...
 * In the Bad Future of Wolverine's "Old Man Logan" arc, Bruce Banner has made a Face Heel Turn and sired the Hulk Gang with his cousin She Hulk.
 * The Sin City short story entitled Daddy's Little Girl has a rather disturbing example of this.
 * The gods are naturally featured in Wonder Woman. Mentioned is their habit of boinking their blood relatives.

Fan Works

 * In Stand In The Rain, a Percy Jackson and The Olympians fanfiction,.
 * It Gets Worse -.
 * Pass the Brain Bleach, please!
 * In the Oneiroi Series, a Order of the Stick series of fanfics, Deirdre
 * Given the fact that most Wizards of Waverly Place fics are focusing on Justin/Alex, many of them portray their relationship as wicked and harmful, not to mention that few have happy endings.
 * In Perfect Lies, Justin has a calm life, a four years old daughter and a loving wife. Then Alex comes and creeps in, destroying it slowly, by alluring him into having sexual affairs with her. And she enjoys it.
 * There is a Pokémon Diamond and Pearl Crack Fic in which Saturn and Jupiter have sex regularly even though they're cousins. Mars also mentions having once had sex with her brother, which she thinks of as okay because the ancient Egyptians used to do it. Granted, none of them actually do anything all that evil, except under Cyrus's orders.
 * Ponies Make War: Titan, having taken over Equestria, attempts to force his daughter Luna to marry her brother Empyrean in order to solidify the latter's position as rightful acting ruler.

Film
"Audience line: "Incest is the best, put your sister to the test!""
 * Eleanor Prentiss Shaw of The Manchurian Candidate (known in the novel as Eleanor Iselin). Both film versions show Eleanor coming onto her son Raymond, whose consent is dubious, and the novel on which it was all based includes frank mentions of . Eleanor figures as a villain in both the novel and the original film version. In the remake, she's a likable character who does horrible things with good intentions, and in all three works the incestuous element is definitely used to enhance her creepiness.
 * Commodus from Gladiator had a sister complex. The sister in question, Luciana, was appropriately horrified.
 * Heavily implied in both versions of Scarface.
 * The Rocky Horror Picture Show: Magenta and Riffraff have a lot of elbow sex.


 * A major plot point in Oldboy.
 * Played for laughs in Blades of Glory
 * Hilarious in Hindsight since the actors are actually married.
 * from Chinatown famously crossed the Moral Event Horizon into Complete Monsterdom when it was revealed that
 * Psycho hints at this, but it's not until the prequel that the subtext becomes a textbook case of Freudian Excuse.
 * In Forrest Gump, Jenny was sexually abused by her father.
 * In Hellraiser, Kristy's Uncle Frank starts tiring of the sex he has with her stepmother. So he decides to turn his attention to Kristy, later noticing how beautiful she's grown up to be, and attempting numerous times to rape her (even culminating in them kissing). Considering that Frank is well known for having broken pretty much all taboos and kinks to have in sex, it's unsurprising that he'd be keen on trying out incest with a minor.
 * Top Dollar and his half-sister Myca from The Crow. They were both into it, they just both happened to be evil.
 * Mother-and-son team of shapeshifting energy vampires from Stephen King's Sleepwalkers. Of course, not being human, they may lack this particular taboo. They're also implied not to have much choice in partners.
 * In Cruel Intentions, Sarah Michelle Gellar's character promises to sleep with her step-brother if he can deflower an avowed virgin before the summer is over.
 * Somewhat averted in that they're not blood relatives, so it's not proper incest.
 * Parodied in Not Another Teen Movie where the interest is reversed with the sister agreeing to help him win the bet to get the nerdy-girl only if he'll agree to have sex with her after he wins. He objects that they are related and she responds "…only by blood!"
 * Implied with the Largos in Repo! The Genetic Opera.
 * The second Hellboy movie had the Big Bad imply that he was in love with his twin sister. Apparently, this was intentional amongst the actors.
 * Parker Posey's character in The House of Yes has this up the wazoo.
 * La Reine Margot (Queen Margot) has this in spades, with the titular Marguerite de Valois having sex with her brothers and their brother Anjou being in love with their mother, Catherine de' Medici. The whole incestuous lot is pretty villainous with atrocities, backstabbing, and poisonings under their belt, though a couple of them are more ambiguous including Margot herself.
 * The 2006 remake of Black Christmas reveals that Billy Lenz's mother despises her own son because of his condition while his father shows kindness. Eventually she decided to kill her husband and locked Billy in the attic so she can have a new life with her new boyfriend. But it turns out her boyfriend is actually impotent after trying to have a new baby with him, so in an effort to have a child she goes to the attic and rapes her 12-year-old son Billy. Nine months later she gives birth to Billy's sister/daughter Agnes (which makes her also the grandmother) and lies to her boyfriend that Agnes is his and mockingly whispers to the eavesdropping Billy that she's her family now.
 * In the Backstory of A Cure for Wellness, Baron von Reichmerl (who changed his name to Dr. Heinreich Volmer) desired to literally have a family of pure blood. He decided that his own sister was suited to carry and give birth to a child. She initially resisted him but the Baron raped her. After some time, she came to accept and even enjoy her relations with her brother and marries him. But unfortunately after several years, the baron realized she was infertile and suspected her body was rejecting the deformed fetus. To cure her infertility, the baron discovered the water of the aquifer which is toxic to humans but had unique life restoring properties to the eels living in the water. He kidnapped several peasants who lived on his land and performed experiments on them by distilling the water and filtering it through their bodies. The process succeeded in "curing" his sister/fiancee’s infertility and she becomes pregnant. At the night of their wedding, the remaining peasants realize the baron was responsible for all the deaths and stormed the castle to capture them and burn them alive. Before his wife/sister was burned, the peasants cut out the baby from her womb and throw it into the aquifer, and the baron was supposedly burned alive himself. Unknown to everyone, he survived and found his child, a girl he named Hannah, and went into hiding. In the film's present time, the baron (now Volmer) immediately marries his daughter Hannah and brings her to a secret room so he can rape her to get pregnant as a way to continue his "pure bloodline". His plan was than thwarted by Lockhart and he was killed by Hannah; his body falls into the aquifer filled with ravenous eels.

Folk Tales

 * A classic fairy tale found throughout Europe concerns, in various variations, a wicked older male relative pursuing a beautiful younger female niece or daughter. Thanks to gender taboos of the period, the fathers never undergo the punishments typical of Grimmification even though the stories otherwise condemn his actions. An even creepier variant, "The Girl with No Hands," is, well, Nightmare Fuel of the worst sort.
 * Peter Stumpp of Bedburg (the original werewolf) was described as having sex with his daughter. The authorities made sure to execute her as well.
 * In some versions of the Arthurian legends Mordred was the son of King Arthur and his half-sister Morgana (swept under the rug in the musical Camelot but dramatized in the movie Excalibur). However, in an inversion of the usual applications of this trope she seduced him (using a disguise spell) as part of her Evil Plan to avenge his father using a similar to have sex with her mother after murdering her father.
 * T.H. White's The Sword in the Stone discusses that it is committing this sin, however unwittingly, that dooms Arthur to such a tragic life.
 * Well, drowning all those children may have had something to do with it, too. While this is usually chalked up as Arthur's greatest sin, it's only one of many sins that bring Camelot down.
 * N.B.: in the original legends his incest was with Morgause, his other half-sister. Modern versions often just combine this role with Morgan(a) for simplicity. (White's version, though, kept Morgause.)
 * In Finnish folktales, the villains, bogeymen and monsters are assumed to have gotten their birth from villainous incest.

Literature

 * The Bible had Lot's daughters raping him, which allegedly produced the Moabites and Ammonites with whom the Israelites had frequent wars. Many consider this a vilification of these rival tribes. Not everyone is entirely sure what happened between Noah and Ham in a certain tent either.
 * Incidentally, Ruth was a Moabite, so the Davidic line also descends from Lot and his daughters.
 * According to some scholars this is the reason for Methuselah Syndrome; inbreeding and the effects of sin have made us the mayflies we are today.
 * Caligula with his sisters and Nero with his mother, both in I, Claudius and (allegedly) in Real Life.
 * Of course the only reason Nero was ever emperor is that his mother married the emperor Claudius. Her uncle. Claudius was the first man in Roman history to marry his niece and had to change the law to permit the union.
 * Agrippina (the niece Claudius married) was also one of Caligula's sisters, by the way. In Paul L. Maier's historical novel The Flames of Rome, Nero's wife chews him out for his part in the affair, specifically pointing out that incest was apparently an old habit with her: "How old was she when her brother Caligula first seduced her, twelve!?" The Julio-Claudian dynasty in its latter years was a major squickfest all around.
 * Ikurei Xerius in Second Apocalypse.
 * In The Silmarillion, Maeglin lusts after his cousin Idril. His feelings are unreciprocated, partly due to the Noldorin incest taboo, partly because she marries someone else. A series of unfortunate events later, Maeglin betrays the location of the formerly hidden city to Morgoth in exchange for lordship and possession of Idril.
 * In The Fall of Numenor, Pharazon becomes the last king of Numenor by marrying the late king's daughter against her will. She's his first cousin. Pharazon's ego and disregard for traditions or basic morality simply made it that much easier for Sauron to corrupt him into Melkor-worship. It should also be noted that Pharazon had no legal right to the throne and marrying Miriel did not actually give it to him. He simply used it as a pretext.
 * There are multiple versions of this. In some of JRRT's versions, Tal-Palantir's daughter is infatuated with Pharazon (who by all accounts was likeable in his youth) and marries him consensually, and hands him the de facto control of the Sceptre. In others, he takes her to himself against her will and seizes the Sceptre with the approval of the general public (who by now were mostly corrupted).
 * Do note that JRRT vacillated a lot on the consensual-nonconsensual nature of sexual relationships in the larger canon; the parents of Maeglin above, for instance, go from Rape Is Love, to totally consensual babymakin', to "wifing by force" (which is Tolkienese for "carry her home and put on your rapin' pants"). In that case the change over time might be partly a race thing; that pairing is, IIRC, the first Noldorin/Sindarin cross and Tolkien was a man of his time. Some fans make a similar argument for the Maeglin/Idril relationship.
 * Celie's father in The Color Purple, although.
 * At one point in Lolita, Humbert Humbert fantasizes about siring a daughter with his adoptive daughter, then a granddaughter with said daughter, and so on. Despite the Misaimed Fandom, Nabokov definitely intended Humbert to cross the Moral Event Horizon.
 * Mason Verger in the book but not The Movie of the Book Hannibal.
 * In his Wold Newton universe, Philip Jose Farmer suggests that Carl Peterson (archfoe of Bulldog Drummond) and his lover Irma (who sometimes posed as his daughter) were, in fact, father and daughter.
 * from A Song of Ice and Fire—both major villains in the Iron Throne plotline. Interestingly, for a long time they were the only genuinely loving couple in the series without a tragic ending in sight. Subverted, in that after Heel Face Turn, he's still really only attracted to his sister, and it's not until she  that the relationship fails.
 * Played straight with the Targaryen family, who married brother-to-sister for hundreds of years to keep their blood pure, which resulted in a degree of being batshit insane. Not all Targaryens qualify as villains, but the ones who do tend to do so in grand style—Aerys II's madness was so violently destructive that he pretty much overthrew his own dynasty, committing several unpleasant murders which sparked off Robert's Rebellion.
 * Also
 * Lord Raith of the White Court in The Dresden Files has the habit of raping his daughters into submission, thus amplifying the evil of this trope by making it non-consensual. He kills his sons for being potential "threats," though, despite the fact that this cuts his potential workforce in half and the canonical fact that the Raiths tend not to care about gender when it comes to food.
 * Thomas says that his father's tastes "don't run that way", and there are a few other times when it's implied that the Raiths do have gender preferences when it comes to feeding, even if they're capable of going both ways
 * Nicodemus and his daughter Deirdre share an kiss, with tongue. Its implied that they've gone farther than that.
 * And The Ass Saw The Angel had an incestuous clan of serial killers living up in the hills. The protagonist's father was a defector from this clan.
 * In The Crying of Lot 49, the characters go to see a Jacobean-era revenge play called The Courier's Tragedy, whose villains are an incestuous family of aristocrats vying for the throne.
 * Terah Graesin and her brother Luc, from the The Night Angel Trilogy.
 * The Ringmaster by David Gurr gets philosophical about this trope—it sets up one of these between a Nazi and his sister, and uses it as something of a metaphor for just how screwed up Those Wacky Nazis were.
 * Paradise Lost uses this as part of an extended allegory of Christian beliefs. Satan has sex with his daughter Sin, who sprang out of his head when he came up with the notion of rebelling against God. Their resulting offspring is Death.
 * Non-consensual incestuous relationships figure in the backstories of several Dean Koontz characters, such as in The Bad Place, Whispers, Life Expectancy, and What the Night Knows.
 * In The Fifth Sorceress, the Face Heel Turn of is made explicit when she makes passes at her captive, though she doesn't actually get a chance to rape him. And also by her becoming bisexual, apparently.
 * In Harry Potter, you can pretty much pick a random member of the Black family and find someone, if not evil, at least highly unpleasant who is married to an awfully close relative, Sirius and Regulus's parents being the most obvious, especially with Walburga screeching about mudbloods and "filth of my flesh." The whole Gaunt family, with the exception of Merope, also fits into this.
 * In the Black family tree the closest couple actually given are second cousins, which is perfectly legal in the UK everywhere - but the close family tree certainly seems designed to highlight the Fridge Logic of insisting on "blood purity". The Gaunts are another matter entirely...
 * In The Elenium, it is fairly common knowledge that the late King Aldreas had a sexual relationship with his sister Arissa, though they weren't so much evil as sickeningly depraved.
 * ...There's a distinction?
 * Yes - it might be disgusting, but given the choice between a King who's porking his sister and a king who's (for example) murdering thousands and skinning puppies, I'd take the incest fellow.
 * The "novelization" of the 1980 Flash Gordon included a small scene of Ming and Aura pleasurably reminiscing about the most recent time they had (BDSM-heavy) sex together. .... eeeeeep.
 * In the Deryni series, this is one of the signs that Imre is a depraved tyrant, though the tyranny is only by medieval standards.
 * The Duet, twin rulers of the Seven Kingdoms in Morgan Llywelyn and Michael Scott's Arcana series.
 * Duchess Alicia clearly lusts after her father and has relations with  and her half-brother.
 * Leo Friend, one of the villains in On Stranger Tides, lusts after Beth Hurwood as a surrogate for his own mother. Technically applies to Beth's father, who plans to oust Beth's spirit from her body so her mother's ghost can move in, although he's so out of touch with reality that the incestuous implications may honestly never have crossed his mind.
 * In the 'Lymond Chronicles by Dorothy Dunnett, the arch-villain.
 * In The Vorkosigan Saga, Richars attempts this on a 12-year-old Lady Donna.
 * Common in the Alex Delaware novels by Jonathan Kellerman.
 * In Mercedes Lackey and James Mallory's The Obsidian Trilogy, Demon Queen Savilla and her son Zyperis frequently engage in sexual activity and express how attractive they find each other.
 * Matthew Lewis both plays it straight and subverts it in The Monk. Played straight when the title character, Ambrosio, driven by his lust for the innocent Antonia  Subverted when he learns
 * Robin McKinley's Deerskin is built on this trope. When his wife dies, he promises to only marry someone as beautiful as she. Should have included a "kin excluded" clause. The rest is nothing short of mortifying.
 * In Red Iron Nights, the serial-killer curse was created by a long-ago evil sorcerer who'd been rejected by an equally-evil sorceress, in favor of her own father.
 * Marik of Gundar never actually has sex with his long-lost (heroic) daughter, but he's not above seducing her into thinking him harmless and attractive with the help of demon-made charms. She doesn't know he's her father at the time, but he definitely thinks it's possible and doesn't even hesitate.

Live Action TV

 * Brother Justin from Carnivale and his sister Iris have a very, er, close relationship with each other. And did we mention he's father of the Anti Christ?
 * And then
 * The X-Files episode "Home."
 * Battlestar Galactica Reimagined: After erasing her true memories, knowingly fraks the scientist who considers herself his mother and created him in the image of her father for extra Squick.
 * The League of Gentlemen. Although it's not revealed until partway through season 2 that Corrupt Hick Serial Killers Tubbs and Edward are brother and sister, it doesn't really come as much of a surprise.
 * Wiseguy. Arms dealers Mel and Susan Profitt.
 * In House of Cards, it's subtly hinted that Francis Urquhart secretly wants to do this. After the elderly Urquhart begins an affair with the young Mattie Storin, and she starts calling him "Daddy," he tells the audience, "I always wanted to be a father of daughters."
 * Divia in Forever Knight, a child vampire who turned her father, proposed this rather forcefully to him. His refusal involved a wooden stake.
 * Blue Heelers pulls this a couple of times, one where a father shoots his own daughter because he wanted to keep her to himself. Another had recurring psycho Luke Darcy rape his own grandmother in his final appearance.
 * On Buffy the Vampire Slayer, part of Spike's back story is that he was pretty much a momma's boy when he was alive, and that after turning into a vampire he spread it to her so that she could live forever. However, losing her soul sort of made her a less friendly person, and she mocked him by claiming that he wanted to go "back inside," trying to seduce him. Spike was so freaked out that he staked her. The fact that she was named Anne, which is also Buffy's middle name, is not coincidental.
 * A more metaphorical example in the Buffyverse would be vampires in general; Darla sired Angelus, and they were a couple (as well as villains) for several hundred years; Angelus sired Drusilla, who calls him "daddy" at one point but also "my Angel" - and they have definitely slept together; Drusilla sired Spike ... Apparently some vampires seek out people to turn into their eternal partner, but since that involves "siring" them, it could be seen as a kind of incest.
 * Spike also refers to Angel as his "grandsire" at one point. And, according to Word of God they have slept together at least once.
 * Added to William and Regan Hamleigh in the miniseries adaptation of The Pillars of the Earth, because apparently they weren't villainous enough already.
 * In a recent (2010) episode of Law and Order Special Victims Unit, a pedophile maintains the perfect cover by leading an activist group that works to expose and shame known sex offenders. It's revealed at the end of the episode that his Start of Darkness was raping his nine-year-old sister when he was a teenager.
 * Jim Profit, Villain Protagonist of Profit has a sexual relationship with his mother, although she is his stepmother and they are not actually related by blood.
 * Vincent/Valerie of Passions, the intersexed child of Julian Crane and Eve Russell, rapes his half-sister twice, has an affair with his uncle Chad, and seduces his own father and becomes pregnant by him. This last sitation prompts the immortal line, "Have your baby so I can get back to drinking," as said by Julian to his child/lover as Vincent delivers their offspring.
 * Game of Thrones: Twins Cersei and Jamie Lannister's sexual relationship is at the heart of much of the plot.

Role Playing Games

 * The Dungeons & Dragons supplement the Book of Vile Darkness introduces the literally Horny Devil Fierna the Lord of the Fourth Layer of the Nine Hells and her father, the former ruler of the Fourth, Belial. Their relationship has continued into the Fourth Edition.
 * Zaiden, the most powerful cleric to the demon lord Yeenoghu, murdered her mother and took her father as her mate. At least for a little while. When she got bored of him she killed him too.
 * In Vampire: The Masquerade, one of the 13 vampire clans are the Giovanni. In addition to being necrophiliac Necromancers and members of The Mafia, they are also one big extended family...and really, really dedicated to "keeping it in the family".
 * Then there are the spiritual descendants, the Sangiovanni bloodline, in Requiem, who are just as devoted to necromancy, necrophilia, and inbreeding.
 * There's an interesting example in Kara no Shoujo. The overall attitude isn't that the incest is inherently wrong (though you may note that there is basically no sexual tension with the protagonists little sister) but that only the mentally unstable would do such a thing.

Theatre

 * Antiochus and his (unnamed) daughter in Pericles. For some reason, her Engagement Challenge is a riddle whose answer is "Antiochus and his daughter are committing incest." They receive a Karmic Death when Apollo blows them up.
 * Shakespeare's Richard III plans to secure himself on the throne by marrying his niece (the daughter of Richard's brother, King Edward IV). In the play, he has his wife killed so that he can do so, although in history she seems to have died of natural causes.
 * For that matter, Hamlet seems to think his paternal uncle marrying his mother counts as incest. Nevermind that's your duty in some cultures.
 * Worth noting that in Shakespeare's England, a man marrying his widowed sister-in-law would have been seen as full-bore incest, about as shocking and scandalous to audiences of that time as Brother-Sister Incest would be to them or us.

Web Comics
"Sidney: Are you two married or brother and sister!? Hansel and Gretel: Yes!"
 * In Fey Winds, two members of Sigurd's Minionz, Hansel and Gretel apply.


 * Yet another rape trope for Drip in Jack. The Anthropomorphic Personification of lust is being punished for his sins in part having to pleasure his grandmother the way he did "when [he was] little." Squick.
 * Later, it's revealed that . He knew the relation, she didn't.
 * Thomas, Nicole's dad in Concession is a supervillain, he also regularly has sex with his son. Though in a strange case the comic shows this as if it's a positive thing.

Video Games

 * Done by the Villain Protagonist of Gibo during the plot, and even has an ending devoted to this.
 * Suggested to have happened between in Wild ARMs 2, and she wasn't exactly consenting to it, either.
 * Shinji Matou of Fate/stay night has repeatedly raped his sister, Sakura. In one of the game's routes, this comes back to haunt him in a big way.
 * The residents of Andale in Fallout 3.
 * BlazBlue resident Jerkass Jin Kisaragi has so much creepy Ho Yay subtext with his brother, Ragna The Bloodedge that it strains the limits of a T-rated game. And if that's not enough, his theme music is titled "Lust Sin."
 * And then in Ragna's ending,
 * One of the major villains in F.E.A.R., Harlan Wade, plays the Evilutionary Biologist and donates his own DNA (along with that of others) for use in impregnating his own daughter in hopes of producing children with powerful psychic abilities. Another villain, Aristide, calls this development 'sick', even though she herself is a Complete Monster.
 * Heavily implied between the twins Alexia and Alfred Ashford, antagonists of Resident Evil - Code: Veronica, especially on Alfred's part.
 * Heavily implied between the antagonists, the Scissortwins, and the Big Bad Dick Hamilton to both his daughter Nancy and granddaughter, the protagonist Alyssa (who looks like her mother), in Clock Tower 3.
 * in House of the Dead: OVERKILL.
 * Ahem, Fire Emblem Genealogy of the Holy War, has, well, this. Alvis and his half sister Diadora. In fact its a major point in the Big Bad's plot to have the two marry and breed, so he could create a vessel for a Dark God.
 * OTOH, other incestuous relationships aren't seen in such bad light. Lachesis was in Unrequited Love with her half-brother Eltoshan, Claud and Sylvia are hinted to be long lost siblings, and they were all good guys. Lester and Lana can marry their cousins Patty and Faval (with the bonus that their moms were identical twin sisters), and they're all on the side of good too.
 * City of Heroes: Tyrant and Dominatrix (Evil Counterparts to Statesman and Ms. Liberty) had Grandparental Incest behind the development team's back. It has since been Ret Conned out of the story, after it was pointed out.
 * It was vaguely hinted at to be happening - largely for Squick reasons - back when the Praetorians were simply Evil Counterparts to the signature heroes. Now that they're morally ambiguous and important characters in their own right, it would be out of place.
 * Used in Assassin's Creed II, which repeats its predecessor's habit of giving each assassination target a Kick the Dog moment just to reassure you it's okay to kill them. This is one of the black marks against the Big Bad Rodrigo Borgia, who is implied to have a sexual relationship with his daughter. Confirmed in the sequel, with the villainy extended to his son, Cesare.
 * In Assassin's Creed Brotherhood Cesare and Lucrezia, the game even opens up with them kissing during the assault on the Assassin's compound. When Cesare is arrested for his crimes, one of them listed is incest.
 * No More Heroes: At the end of the game, Travis (a very dark Anti-Hero, borderline Villain Protagonist ) discovers that
 * In the Interactive Fiction story Anchorhead this is the dark secret of.
 * In Project Justice, Kurow Kirishima pretty much onesidedly lusts after his beautiful older sister Yurika. Made even more obvious in the Darkside Student Council path which is fron Kurow's POV

Western Animation
"Dude, she's your sister!"
 * Jackal from Gargoyles is a Complete Monster who, along with his sister Hyena, is one of the most ridiculously Ax Crazy characters in the Disney canon. However, when Hyena falls in love with Humongous Mecha Coyote, he seems to find it a little more reprehensible than just a normal big brother would, especially considering it's nothing worse than what he's done, and comes off much more as a jealous Stalker with a Crush. He later got most of his body replaced with cyborg parts, and they end up in a pretty sick love triangle for most of the series.
 * ReBoot: While Megabyte's attempt to marry Dot may not seem like incest at first glance, it counts if you understand his origin. He was "born" from an explosion triggered by an experiment gone wrong made by Wellman Matrix (Dot's father), so technically he is Megabyte's father as well. Megabyte even calls the nullified Wellman "father" and Hexadecimal (Megabyte's sister) calls Dot her sister late in the series. So Megabyte marrying Dot falls under this trope.
 * Young Justice Plays With this trope for laughs--Superboy and Miss Martian go undercover as a pair of villainous siblings, though in reality they're Love Interests. When they have their Big Damn Kiss at the end while still in disguise, actual villain Icicle Jr. is horrified.