Butt Monkey/Web Original/Web Animation

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 * Strong Sad from Homestar Runner, whose two brothers Strong Mad and Strong Bad enjoy heaping physical and psychological abuse on him.
 * Also from Homestar Runner, almost everyone in Strong Bad's comic book Teen Girl Squad.
 * Nebulon, Rumble Red, the King of Town, Cartoon Mary Palaroncini, Coach Z, and 1-Up are also frequently abused. There's only one person who does like Nebulon's style... and she's an enormous alien cow. However, it's justified in Red, Mary's and 1-Up's cases, in that they're parodies of annoying cartoon sidekicks.
 * Besides, Red's a communist fool.
 * Homestar Runner himself can also qualify, considering how many times Strong Bad has insulted, assaulted and tricked Homestar for the sake of his own enjoyment. That and Marzipan keeps breaking up with him. Bubs also squeezes Homestar for all the money he can get, but Bubs does that to EVERYBODY.
 * John T. Hedgehog from International Moron Patrol. Issues with John's creator resulted in the series' creator, Roger, reducing John to basically a dumber version of Kenny (though the series explains that John has a guardian angel who revives him at some point after every death. In recent episodes, however, John's abuse has been reduced significantly.
 * Germaine in the Neurotically Yours cartoon series. She's a magnet to stalkers and perverted men, has trouble controlling her weight, is the butt of Foamy's criticisms and Jerkass rage, and can't get any of her poetry published. She recently shaved her hair and gained more weight as a desperate attempt to get men to stop hitting on her. It doesn't.
 * Red vs. Blue. Pretty much all the characters get their turn, but Grif probably qualifies the most, getting yelled at, insulted, threatened, beat up and even shot on occasion, all because he didn't want to risk his life in a war. Oh and because he's snarky.
 * Washington sort of strays into the territory when you consider his speech at the end of Recreation,

"Jaune: I'm tired of being the loveable idiot stuck in a tree while his friends are fighting for their lives. Don't you understand? If I can't do this on my own ... then what good am I?"
 * The title character in the Charlie the Unicorn videos. The blue and pink unicorns apparently come up with extremely convoluted and surreal plans just to make Charlie miserable. This is probably why Charlie is a Deadpan Snarker.
 * Jaune in RWBY, who seems woefully underprepared for the rigors of Beason Academy.   He Lampshaded this at one point: