Action Girl/Web Comics

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In Twice Blessed the pixie Pella Brightwing is a very dangerous combatant who single-handedly holds her own against multiple very tough enemies.
In MAG-ISA, Claudita and Lucia are your standard action girls.
Pirogeth from Megatokyo, Piro's character online/real life in some of the subcomics fits this role pretty
Sal Walters in It's Walky!... unless you catch her on a bad day.
Anne Onymous, Katie McBride, Samantha "Wolfie" Wolf, and Miranda West in The Wotch.
Any female character in Supernormal Step who has plot relevance is likely to be an Action Girl.
All the female main characters in El Goonish Shive (and some of the guys), except for Sarah.
Punchgirl of The Incredible and Awe Inspiring Serial Adventure of The Amazing Plasma-Man.
Cwynhild of Cwynhild's Loom.
Fyre fromThe Beast Legion"
The Continentals' gender bending adventress Lady Fiona Fiziwigg. This men's suited, top hatted, two fisted, gun wielding, say and do as I damned well please agent in her majesty's service is more distressing damsel then damsel in distress as she investigates a series of brutal "mangling" murders uncovers a tangled web of intrigue, adventure and murder with her Continental Operative partner Jeffrey Tiffen Smythe in victorian England. Find it here.
Oasis in Sluggy Freelance, though not an especially heroic one (which is to say, she is completely psychotic). It helps that she doesn't need to worry about being killed, since she's a self-aware satellite or somebody trapped on a satellite or a brain on a satellite or something like that who possesses clones of a particular human, but only one clone at a time.
- Kusari is as much an Action Girl as Oasis, and also possesses clones of herself - but she can possess multiple bodies at once, which lets her be both Kusari and Sasha at the same time.

Zeetha from Girl Genius is a warrior woman from a hidden, jungle city which might possibly have a matriarchal society (this is speculation). She appears to be a stronger warrior than any of the humans in the comic, although she is beaten in combat by the superhuman Baron Wulfenbach.
- There's also that Baron Wulfenbach is perhaps the most badass individual on that planet. Really, losing to him is no shame for anyone.
  - That, and Word of God has confirmed he spent a few years amongst her people and is her father, though neither has realized that last bit.
- The title character is a proficient in making and using death rays and can hold her ground with a sword, but lacks the physique of a real action girl. Fortunately, Zeetha's working on fixing that.
Haley from The Order of the Stick is a high-level chaotic good thief. Her archery is unmatched in the comic. Maybe Vaarsuvius, too.
- In this comic, she defeats a half dozen guards singlehandedly with a weapon she's not even known to be proficient in, in a very short time span.
- Miko might have been a stick-up-the-butt Knight Templar, but this is a woman that managed to solo the Order twice, defeated a minimum 15th level, artifact-enhanced cleric and can behead people with her feet.
- Heck, don't forget Badass Normal / Mama Bear Kazumi. And those poor ninjas thought assassinating a woman who was six-months pregnant would be easy! Also Therkla seems to be one, though we see relatively little of her fighting skill.
- Likewise, Lien (who's so badass that her paladin mount is a shark).
Radical, in Gaming Guardians. Her shapeshifting Evil Twin, Ultima starts out as a Dark Action Girl, but (unusually for the trope) does a Heel Face Turn later -- after having put Radical through seven kinds of hell (including fathering Radical's child while disguised as Randarch, and then causing her miscarriage).
Garanos from the webcomic of the same name.
Juno from Starcross'd Destiny.
Virtually every female character in Errant Story qualifies, though special consideration goes to Sara, the resident warrior-monk. Time-mage warrior-monk.
Jane Mighty (formerly Iron Jane) from Everyday Heroes. She introduced herself to her future husband by kicking him in the head.
The title character of The Challenges of Zona. She and her sister Tula have been known to form a warrior/priestess-mage Lovely Angels duo.
Wendy from Cheshire Crossing.
The Cyantian Chronicles: Silver and Celina best embody this trope. Quinn will, once she does more than spar with family and friends. Chatin, Cilke and a few others cover this trope to a (very slightly) lesser degree.
- It helps that the majority of cyantians have some kind of combat training. Especially the members of the Akaelae family and the Mounty royal family. Which makes sense given the need to protect themselves.
Tonya from Gold Coin Comics is this trope precisely.
Chen-Chen in Harkovast has martial art skills that allow her to block swords (with her bare arms!) and smash skulls (with her bare fists!)
Amy Fang, the Perky Goth heroine of Dead Metaphor has demonstrated Action Girl capabilities while fighting zombies hand-to-hand.
Saffron and Felicia from Fetch Quest: Saga of the Twelve Artifacts. Especially the former.
Tristan in Angel Moxie.
Cassie in Acrobat.
While there are several in Schlock Mercenary -- Legs, 'Chelle, even Breya fit the bill -- Elf takes the cake. Ranging from blowing up a refinery to procto-ing a reality TV host with one of his own cameras.
The Girl from the webcomic A Girl and Her Fed. She's one of the best in the world at Judo, and will use it when necessary.
About half of the cast of Cry Havoc, seeing as they're nearly all mercenaries it's not unexpected.
Tiffany of Exiern, even if she used to be a Proud Warrior Race Guy. Or maybe because of it.
Most female characters of Juathuur are either this or Dark Action Girl. And Never Mess with Granny.
The T-Girls of Jet Dream, all former male Badasses turned Action Girls.
Lucy of Bittersweet Candy Bowl. Most of the male cast have discovered this.
Eve of Bibliography qualifies.
Kira in Panthera not only is a star athlete at school, but also aced Panthera's battle obstacle coure on the first try. Oh, she's a fire-powered tiger that fights against a secret government project with Panthera.
Nonami Mumei from Dr. Nonami can frequently be found battling robots, mad scientists, and other villains.
Rosemary Ripley in The Mansion of E.
Cally from Spy6teen is a Firey Redhead Action Girl.
Rumors of War features both Elysia and Illyra in a seemingly Gun Fu equivalent of the Mysterious Waif. Both have exhibited magical powers that prove useful in close-quarters combat. (Possibly because they're based on Dungeons & Dragons characters.)
Cho from Kagerou fits this trope to a T. Starless also fits as an example.
Elf Blood, being an action webcomic with a majority female cast, would be positively weird without its share of Action Girls. Of note are two of the Incarnum characters: Shanna, a tomboyish brawler; and Carlita, an elegant superspy.
Alex Rayne of Wright As Rayne becomes one after his mind is put into the body of a teenage girl. There are also straight examples in Sareena Black and Ivy League.
Locus from the very NSFW comic of the same name
Kova, of The Player's Guide To S.I.S.U., is easily the toughest member of her team - the first time we meet her, she's duelling one of them, and takes him apart almost without suffering injury herself.
Jessica Queen of Mayonaka Densha is a sword weilding Lady of War who also happens to be a Classy Cat Burglar on the side.
Homestuck: Rose Lalonde, Jade Harley, Kanaya Maryam, Terezi Pyrope, Aradia Megido, Nepeta Lejion, PM, Mom, Nanna, and The Black Queen and Snowman. Basically, if a character is female and in Homestuck, chances are she's this.
Scorpio, Taurus, Aquarius and Sagittarius on the hero side of Zodiac. On the villain side, the female ones are more crafty and seem to prefer to act behind the lines (such as Slaver and Legion), but Nightmare, Basilisk and Razor are straight examples.
In Cucumber Quest, Cucumber's little sister Almond takes swordplay lessons and eagerly volunteers to go on the quest in his place. She later pulls off a Big Damn Heroes moment to save Cucumber.
Comic Hard Graft features all round actiongirl (and madwoman) Bernadette Montez.

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