Supernatural (TV series)/Recap/S01/E20 Dead Man's Blood

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Season 1, Episode 20:

Dead Man's Blood

Sam and Dean travel to Boulder, Colorado after reading about the death of a fellow Hunter. When they arrive, they are reunited with their father and discover that the dead Hunter was in possession of the Colt: an antique handgun forged by Samuel Colt himself, along with thirteen bullets (only a few of which remain) that, when fired from the Colt, are able to kill anything. John wants to use the Colt to kill Yellow Eyes, the creature that killed Mary Winchester, but unfortunately, the Colt is now in possession of the people responsible for the Hunter's death: a coven of vampires. Using the vampires' major weakness (a dead man's blood is a paralytic to them), they capture the coven leader's girlfriend and attempt to trade, but the leader attacks them. During the fight, John gets his hands on the Colt and uses it to kill the leader with a shot to the head.

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