Trigun/Heartwarming

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• Episode 23. Wolfwood's speech gives this troper the sniffles.
• The end of the post-credits scene in the final episode, when Meryl sees Vash come back.
• In the manga, the part in Vash and Knive's past where they discovered the horrors of the previous experiments and, after many days of refusing to eat or touch anything from humans, Rem manages to get through to Vash. Especially how Vash (shown surprisingly to be even more rash and violent than Knives in his youth) tried to kill himself with a knife only for Rem to grab it and get stabbed, with him muttering how he won't feel sad about her death and how he won't cry... only to break down sobbing and holding her.
• "Welcome home."
• The preview for episode 18, which is both heartwarming and tearjerking at the same time:

Kid!Vash: Rem said she came to this ship because someone she loved back on Earth died. I tried to imagine what would happen if Rem died. Suddenly, hot water began to flow from my eyes and didn't stop for a long time. Rem, don't ever leave.

• The end of "Lost July." Doubles as a Tear Jerker.
• Vash's first real smile, in episode 9.