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The artificial intelligence that seems to run Aperture Science. GLaDOS portrays herself as a helpful friend to Chell, but soon unveils a oddly sociopathic side to the testing protocols and a cruel side to her personality. As the game goes on, she becomes more and more sadistic, culminating in a violent confrontation as Chell attempts to escape the testing center. Chell apparently destroys GLaDOS, but at the end of the game she is revealed to have a backup memory and Chell is dragged back to Aperture Science. Years later, Chell and Wheatley unintentionally reboot her. Needless to say, she has a bone to pick with her murderer.
Oh, and she has [[GLaDOS
* [[AcCENT Upon the Wrong SylLABle]]: Done by having Ellen McLain imitate a text-to-speech program's inflections.
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* [[Roaring Rampage of Revenge]]: {{spoiler|Caroline really didn't want to be uploaded}}. As soon as she was turned on, GLaDOS proved completely uncontrollable for the Aperture scientists and had them all killed or placed in suspended animation.
* [[Sarcastic Clapping]]: GLaDOS has an entire processor ''devoted solely to this'', or so she claims.
* [[Self
* [[Shaped Like Itself]]: Her Logic/Intelligence/Knowledge Core, when describing cake garnishes:
{{quote|"Sediment-shaped sediment."}}
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