Me and My Dick/Funny

Joey's little dance and facial expressions after "kissing" Miss Cooter.
When Joey bursts into Sally's room after finding Dick

Sally: I'm dead.

Joey: Oh no you're not. Tell your mom to shut the hell up.

Sally: -to her mom- Shut the hell up, please!

Sally's Mom (offstage): Okay, Sally. I love you!

Joey: Okay, your mom kinda stole my thunder...

Joey proposing to Vanessa:

Joey: I will provide f-

Heart: Louder.

Joey: (Accompanied by weird arm gestures) PROVIDE FOR YOU AND LOVE YOU. YOU WILL KEEP MY HOME AND RAISE MY CHILDREN!!!!

After realizing he's in love with Sally, Joey stammers a couple of seconds them murmurs "Shit!". I think he did the same in A Very Potter Musical.
"JOEY RITCHER IS CRYING IN HERE!"
The entire existence of the character of The Old Snatch. There is just something about the anthropomorphic personification of a vagina as a rugged, crusty old man in a flapper dress and pearls that is simultaneously disgusting and hilarious.