The Prince of Egypt/Tear Jerker

Rameses as he tries to appeal to Moses to stay with him. You can't help but feel sorry for him as he tries virtually everything to keep his brother from leaving Egypt, down to the sadness in his face when Moses does leave.
- In fact, everyone in Moses' adopted family when he finds out where he came from. They are being completely sincere in their love for him, but the things they say to reassure him just drive him further away.
The short scene with Moses and Rameses after the final plague, ending with Moses breaking down and sobbing after.
- From the same scene- listen closely to the ambient noise during this scene. Do you hear that almost inaudible screaming and crying in the background? That's the sound of thousands of Egyptians weeping and wailing over the deaths of their firstborn children. Also may double as Nightmare Fuel, if that kind of thing freaks you out.
Deliver Us, especially the part Ofra Haza sang before the second chrous. This is even exaggerated by the Cantonese lyrics translated here:

My son, even I'm your mother, I have nothing to give
but just a glimmer of hope, that you may be on borrowed time

The plagues. It's so indescribably epic.
When Moses gives Ramses’ ring back. It’s the moment Ramses realizes that nothing can be the same among them again. The worst is how his face changes. From the deep sadness of a man who lost his brother, into the merciless, cold look of the Pharaoh.
"Why can't things be they way they were before?"
After Ramses rejects Moses one final time ("And a great cry shall be heard in Egypt..."), Ramses's son looks at Moses, terrified. Moses looks back, and his expression makes it clear that he knows it's the last time he'll ever seen his nephew alive. Made even worse--Ramses's son is standing just below all the Hebrew babies, sent to drown, engraved on the wall.
That brief moment of focus on Aaron and Miriam when the slavedriver Moses is about to kill first starts beating the old man. Miriam points the cruelty out to her brother, and he immediately turns away to work as intently as possible, with a terrified look on his face. And Aaron in general before the plagues. He's a man who has had his faith trampled into the dust, leaving only fear and bitterness.
- Listen to his voice when Miriam is about to charge at the guard. He's clearly begging her to not do anything to get herself killed.
When Moses inadvertently ends up near the house of Miriam and Aaron. The joy on Miriam's face as she is finally reunited with her brother dissolves into pure heartbreak when she realizes that he thinks of them as just another pair of slaves, especially when he raises his hand to strike her for her 'impudence'.

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