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{{examples}}
== Anime
* In ''[[11eyes]]'', Kakeru has a [[Eyes of Gold|blind right eye]], covered by an [[Eyepatch of Power|an overly large eyepatch]] and a completely normal blue left eye.
* Jio Freed from ''[[666 Satan]]'' has one blue and one red eye, as well as half-and-half hair. This is mainly to symbolize the fact that he [[Super-Powered Evil Side|is a host to Demon 1i, Satan]].
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** His mismatched eyes are also [[Justified Trope|justified]] in a flashback—he lost an eye in a fight years ago, the green one is a transplant.
* In ''[[Black Lagoon]]'', Balalaika is occasionally shown with her right eye glowing yellow-ish.
* [[Cloudcuckoolander|Nenene]] and [[Handsome Lech|Toki]] from ''[[Code Breaker]]'' both have one blue and one brown eye. This almost gets Nenene killed when fire <s>
* {{spoiler|Jeremiah Gottwald}} in ''[[Code Geass]]'' ends up as this after he is made into a cyborg, with one orange eye and the replacement one being green. Arguably, Geass users as well, as when the [[Power Incontinence]] hits, it's usually only in one eye at first, meaning one eye will be its normal color, and the other will have the Geass symbol permanently imprinted on the iris.
* Almost-example: Spike's eyes on ''[[Cowboy Bebop]]'' are slightly different shades of brown. It's very hard, but possible, to notice this before he brings it up to Faye. The reason is that one is a [[Artificial Limbs|cybernetic replacement]] for an eye he lost in a very symbolic and personal tragedy.
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* Urumi Kanzaki from ''[[Great Teacher Onizuka]]'' is heterochromatic and {{spoiler|was conceived via artificial insemination}}.
* Allelujah Haptism from ''[[Mobile Suit Gundam 00]]'' has a [[Gray Eyes]] and a [[Eyes of Gold|gold]] one. His hair always obscures one of the two, cluing any viewers into whether it is he or his evil [[Split Personality]] Hallelujah that is currently in control. {{spoiler|In the end, he ends up pulling his hair back to reveal both eyes, allowing both [[Split Personality Merge|personalities to simultaneously control his body, giving him the reflexes of two people]].}}
* Albireo from ''[[.hack//AI buster
* The [[Artificial Human]] [[Onmyodo|onmyouji]] Abe no Yasuaki and his [[Expy]] Abe no Yasutsugu from the ''[[Harukanaru Toki no Naka de]]'' series both have the light-brown/green combination. [[Fetish Fuel|Somebody must have a thing for this]], since the trend dates back to ''Haruka'''s [[Romance Game|sister franchise]] ''Angelique'' (see above).
** The fifth game in the series seems to be continuing the tradition with Amami (blue and purple). (It's a lot more obvious on Tohko Mizuno's illustration than on the CG image.)
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* Oskar von Reuentahl from ''[[Legend of Galactic Heroes]]'' has one blue and one brown eye, which is the bane of his life -- {{spoiler|it reminded his mother of her illicit affair and drove her to suicide, which caused his father to hate him with a passion.}}
* Rosario from ''[[Lupin III]]: The Columbus Files'' has different colored eyes for plot-relevant reasons. {{spoiler|her left eye was discolored by the laboratory explosion that killed her mother and the peculiar red-brown color allows her estranged [[Mad Scientist]] father to identify her as his child.}}
* Vivio, from ''[[Magical Girl Lyrical Nanoha StrikerS]]
** In ''[[Magical Girl Lyrical Nanoha
* Asuna from ''[[Mahou Sensei Negima]]'' has heterochromia. The exact significance is unknown, but it's especially notable for its ''absence'' in adaptations that don't get into her [[Backstory]].
** {{spoiler|Volume 26's cover has revealed that Arika Entheofushia -- Asuna's relative and Negi's mother -- has/had this as well}}.
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* The Count D from ''[[Pet Shop of Horrors]]'' has heterochromia, one eye being purple and the other, whom he hides under his hair, is golden. In the manga, it's explained that {{spoiler|this is meant to be representative not only for his non-human nature, but also for his inheritance: his father and grandfather have golden and purple eyes, respectively. Since each one has a very different personality, and D has personality traits from ''both'', his heterochromia symbolizes this dual nature}}.
* Ramses from ''[[Red River]]'' has one brown eye and one golden eye.
* In ''[[Rosario
* In ''[[Rozen Maiden]]'' Suiseiseki has red and green eyes, and was originally nicknamed "Boatlights-chan" by fans before she became known as simply "[[Verbal Tic|Desu]]". Her twin sister Souseiseki, introduced later, has the same eye colors but reversed. Appropriately, they are [[foil]]s for each other. Also the previous [[Trope Namer]].
* ''[[Saint Beast]]'': Goh{{context}}
* One of the characters of ''[[Saki (manga)|Saki]]'' has this, and {{spoiler|if Mihoko [[Eyes Always Shut|opens her right eye]] to reveal it to you during a Mahjong game, you could kiss your chances of winning goodbye}}.
* [[Big Bad]] God of War Homura from ''[[Saiyuki]]'' has one [[Eyes of Gold|golden]] eye, and one blue one, as a result of his [[Half-Human Hybrid|father being a human]].
** As does Hakkai when removing his power limiters.
* The perpetually-bandaged Abiru Kobushi from ''[[Sayonara, Zetsubou-sensei]]'' has one brown and one blue eye, though we don't find out until a [[Hot Springs Episode|trip to a bath-house]] temporarily removes the eyepatch.
* Mikuni from ''[[Shangri-La]]'' has one [[Eyes of Gold|gold eye]], and one blue eye.
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* In ''[[Sukisho]]'', whenever Yoru takes over Sora's body, one of his eyes turns gold while the other remains blue.
* In the second novel of ''[[Suzumiya Haruhi]]'', Haruhi gives Mikuru one colored contact to wear during their filmed home movie, because "different colored eyes give an air of mystery". Hey, if anyone [[Genre Savvy|knows her tropes]], it's Haruhi.
** Though anyone watching the first broadcast episode ''alone'' won't know why it was there ''for one shot only''. Other than <s>continuity issues</s> [[A Wizard Did It|Haruhi did it]], it's a case of [[All There in the Manual|All There
* Syaoran of ''[[Tsubasa Reservoir Chronicle]]'' has differently shaded eyes to signify how one is blind. This ''further'' symbolizes how {{spoiler|he only has half a soul, donated to him by the real Syaoran, of whom he is a clone. When the real Syaoran awakens, the Syaoran we've been seeing not only turns evil, but rips out Fai's eye (containing his magic) and eats it, turning his blind eye blue.}}
** By the last chapter of the manga, it's possible (although not confirmed by a color image) that
* [[Big Bad]] (of at least one arc) Rido Kuran of ''[[Vampire Knight]]'' has one blue eye and one crimson eye, a trait that shows up in the people he possesses. This is the first clue to Rima Touya that something's [[Not Himself|not quite right]] about Shiki...
* [[Tyke Bomb]] siblings Segiri and Suika in ''[[Weiss Kreuz]]: Gluhen'' each have one blue eye and one brown eye.
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* In ''[[Rental Magica]]'', Itsuki was in an incident that turned his right eye into something [[Evil Eye|demonic looking]]. Before that, said eye was still an unnatural red.
* In ''[[Another]]'', {{spoiler|there's the reverse-boatlights variant, when we find out that under Misaki's [[Eyepatch of Power]], hides a green clairvoyant glass eye ([[Creepy Doll|from a doll]]), in contrast to her right normal red.}}
== Comics ==
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