The Final Sacrifice/Funny

When Troy shoots Satoris:

Tom: "Ow. You shot my butt! What the hell? You shot me in the butt!"

As Satoris dies and burns:

Crow: "So Mike, when most humans get shot in the butt, they burst into flames?"

Mike: "Well, umm..."

Crow: "Turn around, let's try it."

The "Canada Song"
And of course...

Tom: "You've got mud on your face / Ya big dis-grace / Shovin' those sandwiches into your face, / Singin'"

All: "We will, we will, Rowsdower!"

Tom: "SING IT!!"

The entire sequence with Mike Pipper, especially the Yosemite Sam impressions.

Troy: "You knew my father?"

Servo (as Pipper): "Knew him?! He was delicious!"

From the same sequence:

Troy: What happened to him?

Mike (as Pipper): I jerked him!

"I'd like to sing something from Bone Machine..."
Troy trying to cut himself loose from the ropes:

Mike (as Troy): "I dropped it! ... Which is the sharp side? ... Um, I cut both my wrists! ... Um, I somehow swallowed the knife."

When Rowsdower nearly bashes Troy's head in during a brief case of mistaken identity..

Mike (as Troy): "Wait, it's me!"

Tom Servo (as Rowsdower): "I know!"

"Rowsdower-mobile awaaaaaay!"
"Canadian villain Garth Vader!"
When Troy's walking through a forest of trees looking at the map.

Crow(As Troy): Turn left at the tree. That helps.