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"NOOOOOO! EN-OH-OH-OOOOOOOH!!" —Calculon, Futurama, "Bend Her"

NOOOOOOOOOOOOOOO! —Luke Skywalker, Star Wars Episode V: The Empire Strikes Back

NOOOOOOO!!! —Marshall Phoenix Wright Ace Attorney

Nnnnnggghhhhoooooooooooh!! —Edgeworth Ace Attorney Investigations: Miles Edgeworth

NOOOO! —Socrathetic, on You Know You Read Too Much TV Tropes When

"NO! NO! NOOOOO!! —Froggo, Histeria!

NOOOOOOOOOOOOOOOOO! —Death Kwon Do Instructor, Regular Show

'NOOOOOOOOOOOOOO! —Discord, My Little Pony Friendship Is Magic

NOOOOOOOOOOOO!!!! —Strong Bad, Homestar Runner, "independent"

': NOOOOOOOOO!! —'Mikato, BlazBlue: Continuum Shift Extend

NOOOOOOOO!! —Homer, The Simpsons, "Mayored to the Mob"

'NOOOOOOOOO! —Dragon Age II, when Anders uses a bomb to destroy the Chantry.

NOOOOOOO —Finn, Adventure Time

NOOOOOOOOOOOOOOOOOOOOOOOOOOOOO —Linkin Park, Blackout

"NOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!!! By which I mean I cannot do it." —Mojo Jojo, The Powerpuff Girls, "Mo’ Linguish"