The same MOTHER stream resulted in a number of other "duets" between the two, including one to"Can You Feel the Love Tonight." Though Chugga does get a giggle attack that halts his part, Jon once again serenades him with gusto.
Mario Party
Chance Time of Bowser's Magma Mountain. Thanks to Jon, Chuggaa won the game and swapped stars with Wario. This lead to...
Let's not forget Chugga's rendition of the Jetson's theme: replace, George Jetson with 'Josh Jepson', Judy with 'Jonny' and Jane with 'Tim' and then listen to the reactions of everyone else.
Later in Brawl, he asked if since Tim was playing Princess Zelda, did that make Josh a prince since Tim was his "wife". Apparently because of his infatuation with this song, Chugga ships Josh with Tim now.
If you pay close attention to their second New Super Mario Bros. Wii video, you can hear LOTS of talk like this.
In the 3rd vid Chugga says he can't live without Jon. Admittedly it's because he tends to die when Jon does, though.
When they reach the flagpole depending on where they landed, they'll go "I'm on top/bottom" or complain that they wanted to be on top.
"See, no one's understanding those Fire Ball Yum's or Fire Yum's Balls references we're making 'cause that was all stuff we did in the hotel roomlast night."
"Why are you just saying thrusting over and over again?" "Because it's THRUSTING!"
Episode 15 of NSMB Wii has Jon and Josh invoke this while the former is carrying the latter to safety.
Josh: *faking on the verge of crying* Thank you, Jon!
Jon: *ditto* You're welcome.
Josh: You're such a good friend!
Jon: I'm always there for you!
Josh: Okay!
Jon: Okay!
It also has Chuggaa saying to Jon "What did I ever do to you, honey?" Jon almost ends the video right there.
(Chugga dies when the group reach a ? Block. NCS opens it, releasing a Super Mushroom and 3 Ice Flowers; while Chugga's in the bubble, everyone takes an Ice Flower and Josh gets the mushroom)
Chugga: Thanks a lot! Wha--what are you targeting me for!? What did I ever do to you?
Josh: *to Jon, after Jon nearly throws him off a cliff* You just almost fucked me.
Episode 11. Jon has invinciblity, Chugga doesn't:
Chugga: Can I have a hug so I can have star power?
Jon: No.
Chugga: *fake sad* You never hug me anymore!
Jon: Because you don't deserve it anymore.
Chugga: Aww.
Jon: *overdramatic* I NEVER LOVED YOU TO BEGIN WITH!
Chugga: *fake sobbing* You did not just say that! My life no longer has meaning!
Near the end of episode 10, if you listen carefully, you can hear Jon calling the face Chugga is making "adorable".
Subspace Emissary
Chugga has a screenshot of one of the enemies (looking like he's) sucking off Diddy Kong. Jon questions this.
NCS, in a Final Fantasy IV video, mentions a conversation he and Chugga had while they were staying with Jon in Canada... in bed.
In Episode 8, Chugga insisting that Jon pointed at him and winked, while Jon denies it by saying he was 'just blinking'.
In episode 9 during the 2 dark Samus fight, Emile seems like he wants to see Jon naked.
Chugga says that Zero Suit Samus's name is technically inaccurate, as she's actually wearing a suit. Proton Jon replies to this with "It's not a birthday suit." Chugga brings up that it's Jon's birthday. Hilarity Ensues.
Most likely unintentional, but still funny enough to warrant a mention - Before playing Slot Car Derby on the first board, Chuggaa positions his character's car, and it looks like he's mounting Jon from behind.
During Minigame Coaster, Chugga accidentally kicks Tim's leg and feels something in his pocket. He asks if it's his phone, but it's actually his wallet, and when Jon realizes what's going on he has this line:
Earlier in the Coaster, they get to talking about Jon's clothes. Chugga implies that Jon is wearing fewer clothes than normal. Jon, after making it explicitly clear that he's not naked, then goes on to tell them not to give the shippers more fuel.
Mario Power Tennis Tournament
This exchange at the beginning:
Emile: So, because we have two people named Jon here, we are going to be referring to our Jon as Jon. I own him, and so does Tim.
Tim comments on the method of exchanging food (what appears to be regurgitating food into others mouths) in Episode 2 after Emile shares food with both Jon and Tim. Lampshaded by Jon.
Tim: That was so kinky.
Emile: Thanks for that!
Jon: I was wondering who was going to break the ice about that subject.
